Detailed information of TA system TA152541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3581867..3583088
• Replicon: chromosome
• Accession: NZ_CP050324
• Organism: Pseudomonas aeruginosa strain DVT427

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HB742_RS16385
• Protein ID: WP_003102740.1
• Coordinates: 3582510..3583088 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HB742_RS16380
• Protein ID: WP_003088112.1
• Coordinates: 3581867..3582421 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB742_RS16355	3577435..3577986	+	552	WP_004343115.1	XRE family transcriptional regulator	-
HB742_RS16360	3577983..3578381	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HB742_RS16365	3578394..3578717	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HB742_RS16370	3579048..3579509	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HB742_RS16375	3579514..3581709	+	2196	WP_016263578.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HB742_RS16380	3581867..3582421	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HB742_RS16385	3582510..3583088	+	579	WP_003102740.1	HD domain-containing protein	Toxin
HB742_RS16390	3583197..3584384	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HB742_RS16395	3584374..3586545	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
HB742_RS16400	3586535..3587812	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T152541 WP_003102740.1 NZ_CP050324:3582510-3583088 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T152541 NZ_CP066255:60198-60668 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT152541 WP_003088112.1 NZ_CP050324:3581867-3582421 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT152541 NZ_CP066255:59758-60201 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07