Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 1854599..1856107 Replicon chromosome
Accession NZ_CP050324
Organism Pseudomonas aeruginosa strain DVT427

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag HB742_RS08630 Protein ID WP_003119682.1
Coordinates 1854599..1855186 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9HYX2
Locus tag HB742_RS08635 Protein ID WP_003115566.1
Coordinates 1855253..1856107 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB742_RS08625 1850854..1854333 - 3480 WP_033877375.1 hybrid sensor histidine kinase/response regulator -
HB742_RS08630 1854599..1855186 + 588 WP_003119682.1 GNAT family N-acetyltransferase Toxin
HB742_RS08635 1855253..1856107 + 855 WP_003115566.1 AraC family transcriptional regulator Antitoxin
HB742_RS08640 1856204..1858369 + 2166 WP_003091606.1 TonB-dependent receptor family protein -
HB742_RS08645 1858544..1860418 + 1875 WP_003117607.1 MFS transporter -
HB742_RS08650 1860479..1860688 - 210 WP_003115568.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T152540 WP_003119682.1 NZ_CP050324:1854599-1855186 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T152540 NZ_CP066255:21353-21796 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31933.23 Da        Isoelectric Point: 7.3276

>AT152540 WP_003115566.1 NZ_CP050324:1855253-1856107 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Download         Length: 855 bp

>AT152540 NZ_CP066255:21126-21356 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB Q9HYX2

References