Detailed information of TA system TA152537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3497054..3498275
• Replicon: chromosome
• Accession: NZ_CP050323
• Organism: Pseudomonas aeruginosa strain DVT429

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HB744_RS16535
• Protein ID: WP_003102740.1
• Coordinates: 3497054..3497632 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HB744_RS16540
• Protein ID: WP_003088112.1
• Coordinates: 3497721..3498275 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB744_RS16520	3492330..3493607	+	1278	WP_003102743.1	TolC family protein	-
HB744_RS16525	3493597..3495768	+	2172	WP_009876279.1	type I secretion system permease/ATPase	-
HB744_RS16530	3495758..3496945	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HB744_RS16535	3497054..3497632	-	579	WP_003102740.1	HD domain-containing protein	Toxin
HB744_RS16540	3497721..3498275	-	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HB744_RS16545	3498433..3500628	-	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HB744_RS16550	3500633..3501094	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HB744_RS16555	3501425..3501748	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
HB744_RS16560	3501761..3502159	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HB744_RS16565	3502156..3502707	-	552	WP_003102727.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T152537 WP_003102740.1 NZ_CP050323:c3497632-3497054 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T152537 NZ_CP066254:c4844335-4844051 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT152537 WP_003088112.1 NZ_CP050323:c3498275-3497721 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT152537 NZ_CP066254:c4844567-4844325 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07