152535

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 2147504..2148112 Replicon chromosome
Accession NZ_CP050323
Organism Pseudomonas aeruginosa strain DVT429

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag HB744_RS10060 Protein ID WP_019486378.1
Coordinates 2147765..2148112 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag HB744_RS10055 Protein ID WP_003114155.1
Coordinates 2147504..2147755 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB744_RS10030 2142524..2143132 + 609 WP_003163344.1 hypothetical protein -
HB744_RS10035 2143142..2143492 + 351 WP_003159569.1 DUF2523 domain-containing protein -
HB744_RS10040 2143494..2144756 + 1263 WP_023093179.1 hypothetical protein -
HB744_RS10045 2145015..2146307 + 1293 WP_003123045.1 hypothetical protein -
HB744_RS10050 2146307..2147290 + 984 WP_003114154.1 tyrosine-type recombinase/integrase -
HB744_RS10055 2147504..2147755 + 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HB744_RS10060 2147765..2148112 + 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HB744_RS10065 2148421..2149329 - 909 WP_003141085.1 LysR family transcriptional regulator -
HB744_RS10070 2149562..2150713 - 1152 WP_012761372.1 trypsin-like peptidase domain-containing protein -
HB744_RS10075 2150738..2151703 - 966 WP_046238418.1 M48 family metalloprotease -
HB744_RS10080 2151681..2152178 - 498 WP_020306406.1 heat resistance protein PsiE-GI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2135760..2224864 89104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T152535 WP_019486378.1 NZ_CP050323:2147765-2148112 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T152535 NZ_CP066254:4127407-4127625 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT152535 WP_003114155.1 NZ_CP050323:2147504-2147755 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT152535 NZ_CP066254:4127006-4127380 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References