Detailed information of TA system TA152518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2697376..2699171
• Replicon: chromosome
• Accession: NZ_CP050313
• Organism: Shewanella aestuarii strain PN3F2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HBH39_RS11860
• Protein ID: WP_167680065.1
• Coordinates: 2697834..2699171 (-)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HBH39_RS11855
• Protein ID: WP_167678515.1
• Coordinates: 2697376..2697837 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBH39_RS11825	2693481..2693747	+	267	WP_167678506.1	DUF1778 domain-containing protein	-
HBH39_RS11830	2693738..2694244	+	507	WP_167678508.1	GNAT family N-acetyltransferase	-
HBH39_RS11835	2694386..2695924	-	1539	WP_167678510.1	Fic family protein	-
HBH39_RS11840	2696045..2696122	-	78	Protein_2284	integrase	-
HBH39_RS11845	2696177..2696893	+	717	WP_167678512.1	hypothetical protein	-
HBH39_RS11850	2696847..2697290	+	444	WP_167678514.1	hypothetical protein	-
HBH39_RS11855	2697376..2697837	-	462	WP_167678515.1	helix-turn-helix domain-containing protein	Antitoxin
HBH39_RS11860	2697834..2699171	-	1338	WP_167680065.1	HipA domain-containing protein	Toxin
HBH39_RS11865	2699748..2700848	+	1101	WP_167678517.1	Fic family protein	-
HBH39_RS11870	2701096..2701197	-	102	WP_167680066.1	IS66 family insertion sequence element accessory protein TnpB	-
HBH39_RS11875	2701201..2701320	-	120	WP_167678519.1	IS66 family insertion sequence element accessory protein TnpB	-
HBH39_RS11880	2701268..2701622	-	355	Protein_2292	helix-turn-helix domain-containing protein	-
HBH39_RS11885	2701688..2703709	-	2022	WP_167678521.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2687292..2700848	13556
	-	flank	IS/Tn	-	-	2701353..2701622	269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (163-389)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 50759.51 Da
• Isoelectric Point: 4.5315

>T152518 WP_167680065.1 NZ_CP050313:c2699171-2697834 [Shewanella aestuarii]
MEHLTIQAFINGEWRDIAQLSFPESEQGNYRLTSLDYEQDYAVAELGKDDIHAVSINHPVSLFFDTPAWLHFLDDIMPSG
ASRRYWLKYLDIADLSVDEQNFVLLKYGTIAPIGNLRVKESLPERLAISDELMFTVEDVKNRASDFLDYAQHRGAAAGGA
TGAGGEAPKLLLRCDDEQNIWIDSYQDEPQNQDLYYLVKYPRGSRSPIDCDILRAEYHFYHELAEMGFETIPIANMLLEE
GHSYPSLWLPRFDVYFNERGQQRYSMESVYSMLGKGAGVTLDHEQTIRALIDKITQSYMVIEQGYQFDLRAFVIEWVQRD
LLNILFGNSDNHGRNTAFLRGDGWIKLAPIYDFAPMKADPEGVARTTKWQAPLEVGGVYDFPGIAEALADLVPAKELMTE
LKLTAQQCLDLKQRLIARSVPKQIINMPSIGFTYIEDKLTRWGLL

Nucleotide

• Length: 1338 bp

>T152518 NZ_CP066249:c4908376-4908092 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16937.48 Da
• Isoelectric Point: 10.9902

>AT152518 WP_167678515.1 NZ_CP050313:c2697837-2697376 [Shewanella aestuarii]
MSDKSTITETAKETIARLRANRQQRSELEKEKSQPHQTRGVEEAQTKQGAKRASKQARGVSTVQRKKAISAIAQKLLHGE
LTQGQALKALRVEVLGLTQDEYASLAKVSRKTLSDVENDRGSFKTDILDRLFKPFALKVGIVPITIGVTTRIL

Nucleotide

• Length: 462 bp

>AT152518 NZ_CP066249:c4908608-4908366 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA