Detailed information of TA system TA152486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 126773..127396
• Replicon: chromosome
• Accession: NZ_CP050301
• Organism: Phyllobacterium sp. 628

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HB779_RS02100
• Protein ID: WP_188344175.1
• Coordinates: 126773..127069 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HB779_RS02105
• Protein ID: WP_188344176.1
• Coordinates: 127076..127396 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB779_RS02065	121890..122171	-	282	WP_188344168.1	hypothetical protein	-
HB779_RS02070	122174..122863	-	690	WP_188344169.1	hypothetical protein	-
HB779_RS02075	122877..123164	-	288	WP_188344170.1	hypothetical protein	-
HB779_RS02080	123161..124717	-	1557	WP_188344171.1	hypothetical protein	-
HB779_RS02085	124714..125106	-	393	WP_188344172.1	hypothetical protein	-
HB779_RS02090	125215..125859	-	645	WP_188344173.1	recombinase family protein	-
HB779_RS02095	126088..126600	+	513	WP_188344174.1	hypothetical protein	-
HB779_RS02100	126773..127069	+	297	WP_188344175.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB779_RS02105	127076..127396	+	321	WP_188344176.1	putative addiction module antidote protein	Antitoxin
HB779_RS02110	127777..128079	+	303	WP_188344177.1	HigA family addiction module antidote protein	-
HB779_RS02115	128072..128320	-	249	WP_188344178.1	DNA-binding protein	-
HB779_RS02120	128317..128616	-	300	WP_188344179.1	hypothetical protein	-
HB779_RS02125	128697..129233	-	537	WP_188344180.1	hypothetical protein	-
HB779_RS02130	129230..129421	-	192	WP_188344181.1	hypothetical protein	-
HB779_RS02135	129414..129656	-	243	WP_188344182.1	hypothetical protein	-
HB779_RS02140	129653..129871	-	219	WP_188344183.1	hypothetical protein	-
HB779_RS02145	129868..130092	-	225	WP_188344184.1	hypothetical protein	-
HB779_RS02150	130181..130372	-	192	WP_188344185.1	hypothetical protein	-
HB779_RS02155	130507..130893	+	387	WP_188344186.1	Arc family DNA-binding protein	-
HB779_RS02160	130942..132192	+	1251	WP_188344187.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	121890..130893	9003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-91)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11115.66 Da
• Isoelectric Point: 8.8496

>T152486 WP_188344175.1 NZ_CP050301:126773-127069 [Phyllobacterium sp. 628]
MVEVRQTDVYSTWFAKLKDHNARSRINVRIRRLELGNPGKVEPVGEGVSELKIDYGPGYRVYYINRGNTVVILLCGGDKS
TQDEDIKTAKQMASEIKE

Nucleotide

• Length: 297 bp

>T152486 NZ_CP066215:497406-497747 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11462.90 Da
• Isoelectric Point: 4.1203

>AT152486 WP_188344176.1 NZ_CP050301:127076-127396 [Phyllobacterium sp. 628]
MAVIINKWDVTEHLESEEAIFSYINAALEDGDPSLISAAVGDVARARGMTQIAKDTGLSRESLYRSLGVGGNPEFATLLK
VFQALGIQLKAEPTHEVDQPDMVDCD

Nucleotide

• Length: 321 bp

>AT152486 NZ_CP066215:497176-497406 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG