Detailed information of TA system TA152474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 908694..909711
• Replicon: chromosome
• Accession: NZ_CP050296
• Organism: Mesorhizobium huakuii strain 583

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HB778_RS04495
• Protein ID: WP_183464996.1
• Coordinates: 908694..909272 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: HB778_RS04500
• Protein ID: WP_183461822.1
• Coordinates: 909268..909711 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB778_RS04470	904125..904610	-	486	WP_183461814.1	hypothetical protein	-
HB778_RS04475	904831..905766	-	936	WP_183461816.1	tetratricopeptide repeat protein	-
HB778_RS04480	905706..906812	-	1107	WP_183461818.1	tetratricopeptide repeat protein	-
HB778_RS04485	907040..908155	-	1116	WP_183461820.1	alkene reductase	-
HB778_RS04490	908199..908489	-	291	WP_013894257.1	helix-turn-helix domain-containing protein	-
HB778_RS04495	908694..909272	-	579	WP_183464996.1	PIN domain-containing protein	Toxin
HB778_RS04500	909268..909711	-	444	WP_183461822.1	helix-turn-helix domain-containing protein	Antitoxin
HB778_RS04505	909935..910633	-	699	WP_183461824.1	RES family NAD+ phosphorylase	-
HB778_RS04510	910627..911037	-	411	WP_183461826.1	MbcA/ParS/Xre antitoxin family protein	-
HB778_RS04515	911104..911541	-	438	WP_183461828.1	hypothetical protein	-
HB778_RS04520	911621..913054	-	1434	WP_183461830.1	aldehyde dehydrogenase family protein	-
HB778_RS04525	913231..913614	-	384	WP_183461832.1	ACT domain-containing protein	-
HB778_RS04530	913642..914109	-	468	WP_183464997.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-116)

Antitoxin

• SinI-like, DNA-binding domain (77-121)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21735.10 Da
• Isoelectric Point: 5.7432

>T152474 WP_183464996.1 NZ_CP050296:c909272-908694 [Mesorhizobium huakuii]
IKTSPYTAVLDACVLYPAPLRDLLLELAAGGSLYRAKWTEDIHDEWTRNLAKDRPDLKPEQIARTRDLMNRSVPDCLVEG
YQELIPSLTLPDENDRHVRAAAIVSAADAIVTFNLKDFPKPVLDKYLLDLIHPDEFIFNQFGINDAAVLMSAQRCRLRLK
NPPKTVSEYLDMLEMQGLPKTVSRLRTYAGVI

Nucleotide

• Length: 579 bp

>T152474 NZ_CP066187:3487113-3487853 [Bacillus cytotoxicus]
TTGACTGTCACTAGCGTTCGAATTGGCTTGTTTTTTCTTGTAATCTTTTTTTTATTTCTTATTTTCTTTTATTGGAGAAA
TGAAGAACGATACGAGGAAAGAAAACAACTTATTCGAAAGACGTGGTATGGTTTATTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAACATATTTTAATGGTTGTTGCGATGGTTGTATTTGTTGATATTGCA
TTTATTTTAACGCCAAATATTTCGGAAATCTGGGGCGCAAAGTTTAGTGATATTGGTAAGACAGTTCAATCGATTAAACG
ATCGCTTATTGCTTCAAAAGCAAGAGGAGAAATATATACAACACTTATTCAAAATGTAAATCCAGCTGCATTTGGTACGA
TGGAATGGGAAACAGATGCTGAGTATGCACGCAGTTTAAATGCTTTTCTTGATACGTATGCTGAAAAAATAGGTGCGAAA
ATTGTAGTATTTGTCACAGAAGAGGAATTAAATACAAACTTCCGAGGTGTTCGATCTCAATTTAGTATTACAGTATCACC
CGAATCTATTGAACAACTGAATGAGCAAAAAGCAGTTCAAATTGAAAATGTTGGAATTATACCAGCGAAAATAATAAAAG
ACGTTTATATTATTATGGATGGACAAAAAAATAATTTGCAAGATCGTGACTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTACTTTTGTAAGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16221.62 Da
• Isoelectric Point: 6.4872

>AT152474 WP_183461822.1 NZ_CP050296:c909711-909268 [Mesorhizobium huakuii]
MPRLLERPILPSADDAELAAQASRQLSLGKHAGAELRVQVGNETLRLPKSVGDLLCHLLTEMGQGNAVTIIPVHAELTTQ
EAADYLNVSRPYLIRLLEEGKLPFNMVGTHRRVKFSDLATYRSSVEDGRKRVMEELAAQAQELGMGY

Nucleotide

• Length: 444 bp

>AT152474 NZ_CP066187:3487977-3488105 [Bacillus cytotoxicus]
GTGTTAAAAGAATATAGCAATGATTTCTTTAAAGACGAAGCTGAGGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTGGAAGAATTAGAAGAAGAATTGAAAAGAAGAGGATAA