Detailed information of TA system TA152472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 361855..362621
• Replicon: chromosome
• Accession: NZ_CP050296
• Organism: Mesorhizobium huakuii strain 583

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HB778_RS01715
• Protein ID: WP_183464955.1
• Coordinates: 361855..362340 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: HB778_RS01720
• Protein ID: WP_183460970.1
• Coordinates: 362340..362621 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB778_RS01695	357909..358936	+	1028	Protein_334	sulfate ABC transporter substrate-binding protein	-
HB778_RS01700	359018..359869	+	852	WP_183460964.1	sulfate ABC transporter permease subunit CysT	-
HB778_RS01705	359862..360746	+	885	WP_183460966.1	sulfate ABC transporter permease subunit CysW	-
HB778_RS01710	360760..361812	+	1053	WP_183460967.1	sulfate/molybdate ABC transporter ATP-binding protein	-
HB778_RS01715	361855..362340	-	486	WP_183464955.1	GNAT family N-acetyltransferase	Toxin
HB778_RS01720	362340..362621	-	282	WP_183460970.1	DUF1778 domain-containing protein	Antitoxin
HB778_RS01725	362864..363850	+	987	WP_183460972.1	sulfate ABC transporter substrate-binding protein	-
HB778_RS01730	364139..364549	+	411	WP_183460974.1	Rrf2 family transcriptional regulator	-
HB778_RS01735	364632..365762	+	1131	WP_183460976.1	hypothetical protein	-
HB778_RS01740	365757..366305	-	549	WP_183460978.1	gluconokinase	-
HB778_RS01745	366308..367258	-	951	WP_183464956.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	356710..357276	566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-77)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17102.72 Da
• Isoelectric Point: 8.8804

>T152472 WP_183464955.1 NZ_CP050296:c362340-361855 [Mesorhizobium huakuii]
MLSAPTPLAENHDLELFQSGTESLDQWLRRRARANQVGGASRTYVVAEGPRVVGYYCLSSGGLDLAEAPGSIRRNMPDPI
PMVVLGRLAVDAGWQGKGLGAALLQDAVLRAGNAAMILGIRGIFVHAISDEAKAFYEHYGFAASPKNPMTLVLSLKGLVK
G

Nucleotide

• Length: 486 bp

>T152472 NZ_CP066185:3094949-3095689 [Bacillus cytotoxicus]
TTGACTGTCACTAGCGTTCGAATTGGCTTGTTTTTTCTTGTAATCTTTTTTTTATTTCTTATTTTCTTTTATTGGAGAAA
TGAAGAACGATACGAGGAAAGAAAACAACTTATTCGAAAGACGTGGTATGGTTTATTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAACATATTTTAATGGTTGTTGCGATGGTTGTATTTGTTGATATTGCA
TTTATTTTAACGCCAAATATTTCGGAAATCTGGGGCGCAAAGTTTAGTGATATTGGTAAGACAGTTCAATCGATTAAACG
ATCGCTTATTGCTTCAAAAGCAAGAGGAGAAATATATACAACACTTATTCAAAATGTAAATCCAGCTGCATTTGGTACGA
TGGAATGGGAAACAGATGCTGAGTATGCACGCAGTTTAAATGCTTTTCTTGATACGTATGCTGAAAAAATAGGTGCGAAA
ATTGTAGTATTTGTCACAGAAGAGGAATTAAATACAAACTTCCGAGGTGTTCGATCTCAATTTAGTATTACAGTATCACC
CGAATCTATTGAACAACTGAATGAGCAAAAAGCAGTTCAAATTGAAAATGTTGGAATTATACCAGCGAAAATAATAAAAG
ACGTTTATATTATTATGGATGGACAAAAAAATAATTTGCAAGATCGTGACTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTACTTTTGTAAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10677.22 Da
• Isoelectric Point: 6.4712

>AT152472 WP_183460970.1 NZ_CP050296:c362621-362340 [Mesorhizobium huakuii]
MTKAVPETHAKPVNLRIREDIRILIDRAAKLRGKTRSDFMIDAAYRAAEDTLLDQTLIKVDPESYRHYLDILDEPPSGEG
FARLMNAPKPWQD

Nucleotide

• Length: 282 bp

>AT152472 NZ_CP066185:3095813-3095941 [Bacillus cytotoxicus]
GTGTTAAAAGAATATAGCAATGATTTCTTTAAAGACGAAGCTGAGGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTGGAAGAATTAGAAGAAGAATTGAAAAGAAGAGGATAA