Detailed information of TA system TA152467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 976083..976672
• Replicon: chromosome
• Accession: NZ_CP050291
• Organism: Pseudomonas plecoglossicida strain PQLYC4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HAV28_RS04360
• Protein ID: WP_028625322.1
• Coordinates: 976083..976379 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: S2KHC3
• Locus tag: HAV28_RS04365
• Protein ID: WP_016393973.1
• Coordinates: 976376..976672 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAV28_RS04335	971705..972559	+	855	WP_016393967.1	putative selenate ABC transporter substrate-binding protein	-
HAV28_RS04340	972556..973350	+	795	WP_016393968.1	phosphonate ABC transporter ATP-binding protein	-
HAV28_RS04345	973344..974171	+	828	WP_016393969.1	ABC transporter permease	-
HAV28_RS04350	974168..974935	+	768	WP_016393970.1	phosphonate ABC transporter, permease protein PhnE	-
HAV28_RS04355	974970..975860	-	891	WP_016393971.1	hypothetical protein	-
HAV28_RS04360	976083..976379	+	297	WP_028625322.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HAV28_RS04365	976376..976672	+	297	WP_016393973.1	putative addiction module antidote protein	Antitoxin
HAV28_RS04370	976678..977163	-	486	WP_016393974.1	GNAT family N-acetyltransferase	-
HAV28_RS04375	977247..977894	-	648	WP_028625324.1	hypothetical protein	-
HAV28_RS04385	978359..979408	+	1050	WP_016393976.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
HAV28_RS04390	979423..980538	+	1116	WP_016393977.1	tRNA guanosine(34) transglycosylase Tgt	-
HAV28_RS04395	980582..980917	+	336	WP_008092587.1	preprotein translocase subunit YajC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (41-87)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10910.84 Da
• Isoelectric Point: 10.9027

>T152467 WP_028625322.1 NZ_CP050291:976083-976379 [Pseudomonas plecoglossicida]
MICVVEQTEHFARWLAGLKDLRAKLAVGRRIERAAMGNLGDFKSVGGSISELRVDVAGGYRIYFTRKQHRLIILLVGGDK
SSQATDILKARKLAKELK

Nucleotide

• Length: 297 bp

>T152467 NZ_CP066179:c444219-443869 [Bacillus cytotoxicus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCAGCGATTACTGCACAGATTCAGAAAG
CAAAATTGCCCACGCATGTGGAGATTGATGCGAAGAAGTACGGATTTGAGAGGGATTCTGTGATCTTGCTCGAGCAGATT
CGAACAATTGATAAGCAACGCTTAACGGACAAAATCACACATTTAGATGAAGTGATGATGATTCGTGTAGATGAGGCTTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10536.12 Da
• Isoelectric Point: 5.6706

>AT152467 WP_016393973.1 NZ_CP050291:976376-976672 [Pseudomonas plecoglossicida]
MNEKLIPFDMVTLLDSDEAISEYLSQVLADGDTDEIIRALGHIARARGMSQIAAGSGLGRESLYKALTPGAKPRFDTVLK
VIRALGIDLSARPHLVSS

Nucleotide

• Length: 297 bp

>AT152467 NZ_CP066179:c444511-444224 [Bacillus cytotoxicus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAGTGGTGACGGAATTGGACGGGATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAAAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: S2KHC3