Detailed information of TA system TA152466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 645365..646008
• Replicon: chromosome
• Accession: NZ_CP050291
• Organism: Pseudomonas plecoglossicida strain PQLYC4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: S2JTH3
• Locus tag: HAV28_RS02880
• Protein ID: WP_016395192.1
• Coordinates: 645365..645778 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: S2KM81
• Locus tag: HAV28_RS02885
• Protein ID: WP_016395191.1
• Coordinates: 645778..646008 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAV28_RS02855	642337..642768	-	432	WP_016395197.1	DUF2946 domain-containing protein	-
HAV28_RS02860	642795..643280	-	486	WP_016395196.1	copper chaperone PCu(A)C	-
HAV28_RS02865	643329..643718	-	390	WP_016395195.1	DUF2946 domain-containing protein	-
HAV28_RS02870	643925..644779	-	855	WP_016395194.1	SPFH/Band 7/PHB domain protein	-
HAV28_RS02875	644807..645244	-	438	WP_016395193.1	NfeD family protein	-
HAV28_RS02880	645365..645778	-	414	WP_016395192.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HAV28_RS02885	645778..646008	-	231	WP_016395191.1	antitoxin	Antitoxin
HAV28_RS02890	646098..646817	-	720	WP_016395190.1	cobalt-precorrin-6A reductase	-
HAV28_RS02895	646814..647908	-	1095	WP_016395189.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
HAV28_RS02900	647901..649112	-	1212	WP_016395188.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
HAV28_RS02905	649230..650546	+	1317	WP_016395187.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15369.69 Da
• Isoelectric Point: 6.9793

>T152466 WP_016395192.1 NZ_CP050291:c645778-645365 [Pseudomonas plecoglossicida]
MLRYMLDTNICILTIKNKPQVVREAFNRHHGQMAISTVTLMELIYGAEKSAHPERNLSIVESFAARLEVLDYDSRAAEHS
GQLRAELAMAGTPIGPFDQLIAGHARARGLVLVTNNLREFQRVPGLRMEDWLDTPTA

Nucleotide

• Length: 414 bp

>T152466 NZ_CP066174:c188183-187701 [Klebsiella quasipneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGATGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGAAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8409.28 Da
• Isoelectric Point: 4.2545

>AT152466 WP_016395191.1 NZ_CP050291:c646008-645778 [Pseudomonas plecoglossicida]
MDQGAVFKSNRSQAIRLPKSVALPEEVTRVDIVAVGRARIITPAGESWDTWFDGDDASPDFMASRDQPADQEREGF

Nucleotide

• Length: 231 bp

>AT152466 NZ_CP066174:c188452-188174 [Klebsiella quasipneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACACCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: S2JTH3

Antitoxin

• AlphaFold DB: S2KM81