Detailed information of TA system TA152465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 293551..295280
• Replicon: chromosome
• Accession: NZ_CP050291
• Organism: Pseudomonas plecoglossicida strain PQLYC4

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: HAV28_RS01380
• Protein ID: WP_028625067.1
• Coordinates: 293551..294192 (+)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: S2K0E1
• Locus tag: HAV28_RS01385
• Protein ID: WP_016393333.1
• Coordinates: 294192..295280 (+)
• Length: 363 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAV28_RS01360	289647..290162	+	516	WP_016393328.1	dihydrofolate reductase	-
HAV28_RS01365	290199..291590	-	1392	WP_016393329.1	L-cystine transporter	-
HAV28_RS01370	291832..292887	+	1056	WP_016393330.1	haloacid dehalogenase-like hydrolase	-
HAV28_RS01375	292976..293554	+	579	WP_016393331.1	DUF4433 domain-containing protein	-
HAV28_RS01380	293551..294192	+	642	WP_028625067.1	DUF4433 domain-containing protein	Toxin
HAV28_RS01385	294192..295280	+	1089	WP_016393333.1	macro domain-containing protein	Antitoxin
HAV28_RS01390	295339..297180	-	1842	WP_016393334.1	dihydroxy-acid dehydratase	-
HAV28_RS01395	297501..298577	+	1077	WP_043172821.1	phosphatidylinositol-specific phospholipase C	-
HAV28_RS01400	298823..299878	+	1056	WP_016393336.1	magnesium transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	131482..380342	248860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24628.20 Da
• Isoelectric Point: 9.1292

>T152465 WP_028625067.1 NZ_CP050291:293551-294192 [Pseudomonas plecoglossicida]
MIYSSLNPSKALIWRIVHRDNLPWLLDHGLHAVSSGRMSSTYVGIGNPELISKRAQRDVPIEPNGTLADYVPFYFTPFSV
MMKNIHSGWSVQQRRNEEIVILVSSLYHAQSLGLRFVFTDAHAYPHWTGYYSDLAQLDKIDWEILQRRDFKRDPDDPRKM
ERYQAEALIHGHVPVAGLLGIVCYNEALKSRVVKEVQQRQLSLPVHARPGWYF

Nucleotide

• Length: 642 bp

>T152465 NZ_CP066174:c144681-144238 [Klebsiella quasipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 363 a.a.
• Molecular weight: 40514.55 Da
• Isoelectric Point: 5.8940

>AT152465 WP_016393333.1 NZ_CP050291:294192-295280 [Pseudomonas plecoglossicida]
MIRFMQGNLLEAKADALVNTVNTVGVMGKGIALMFKERFTENYRQYAAACKAKEVVTGKMFVTEVRELDGPRWIVNFPTK
RHWRSPSQIGWIVDGLHDLRSFIIENQVVSIAIPPLGAGNGGLPWTAVREQIESILKDLDADILVYEPTANYQNVSKRSG
VENLTPARALIAELIRRYWVLGMECSLLEVQKLAWFLERSIERFTPGVNPLDLKFVAYKYGPYANRLDHLLDNLDGSYLH
CGKRISDAGPLDVIWFDEGRKAFVQAYLNSEAKVHLPALEFTADLIDGFESPFGMELLATVDWLLHEDGVEPTVPAVREH
LKNWPGGAGAAARKSKLFDDDSLGIALEKLSMFRSDPAASLH

Nucleotide

• Length: 1089 bp

>AT152465 NZ_CP066174:c144908-144678 [Klebsiella quasipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: S2K0E1