Detailed information of TA system TA152435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 51669..52342
• Replicon: plasmid unnamed1
• Accession: NZ_CP050261
• Organism: Pseudomonas coronafaciens strain X-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q52562
• Locus tag: HBB04_RS26140
• Protein ID: WP_005769733.1
• Coordinates: 51669..52088 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HBB04_RS26145
• Protein ID: WP_024671647.1
• Coordinates: 52085..52342 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBB04_RS26120	48180..48836	+	657	WP_157973363.1	DUF2971 domain-containing protein	-
HBB04_RS26125	48931..49536	-	606	WP_080268302.1	histidine phosphatase family protein	-
HBB04_RS26130	49776..50711	-	936	WP_191867947.1	syringolide biosynthetic protein AvrD1	-
HBB04_RS26135	50996..51628	-	633	WP_024671646.1	recombinase family protein	-
HBB04_RS26140	51669..52088	-	420	WP_005769733.1	type II toxin-antitoxin system VapC family toxin	Toxin
HBB04_RS26145	52085..52342	-	258	WP_024671647.1	Arc family DNA-binding protein	Antitoxin
HBB04_RS26150	52692..53348	+	657	WP_017684244.1	hypothetical protein	-
HBB04_RS26155	53396..53650	+	255	WP_024671648.1	hypothetical protein	-
HBB04_RS26160	54035..54574	+	540	WP_024671649.1	hypothetical protein	-
HBB04_RS26165	54834..55475	+	642	WP_191867948.1	SOS response-associated peptidase	-
HBB04_RS26170	55850..56098	+	249	WP_055004694.1	hypothetical protein	-
HBB04_RS26175	56145..56405	-	261	WP_191867949.1	hypothetical protein	-
HBB04_RS26180	56467..56700	+	234	WP_024669557.1	hypothetical protein	-
HBB04_RS26185	56713..56964	+	252	WP_019331343.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..94384	94384

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15138.43 Da
• Isoelectric Point: 4.7010

>T152435 WP_005769733.1 NZ_CP050261:c52088-51669 [Pseudomonas coronafaciens]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Nucleotide

• Length: 420 bp

>T152435 NZ_CP066151:c4824231-4823698 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9415.65 Da
• Isoelectric Point: 9.0330

>AT152435 WP_024671647.1 NZ_CP050261:c52342-52085 [Pseudomonas coronafaciens]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGTFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Nucleotide

• Length: 258 bp

>AT152435 NZ_CP066151:c4824508-4824242 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: Q52562