Detailed information of TA system TA152431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2688132..2688759
• Replicon: chromosome
• Accession: NZ_CP050258
• Organism: Erwinia amylovora strain FB-86

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: EHW96_RS12105
• Protein ID: WP_004156337.1
• Coordinates: 2688541..2688759 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EHW96_RS12100
• Protein ID: WP_013035913.1
• Coordinates: 2688132..2688521 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHW96_RS12080	2683151..2686297	+	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit	-
EHW96_RS12085	2686445..2686726	+	282	WP_004156350.1	type B 50S ribosomal protein L31	-
EHW96_RS12090	2686714..2686860	+	147	WP_004156349.1	type B 50S ribosomal protein L36	-
EHW96_RS12095	2687632..2687985	+	354	WP_004156342.1	hypothetical protein	-
EHW96_RS12100	2688132..2688521	+	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
EHW96_RS12105	2688541..2688759	+	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
EHW96_RS12115	2689059..2689376	+	318	WP_004156334.1	MGMT family protein	-
EHW96_RS12120	2689399..2689956	-	558	WP_004156333.1	YbaY family lipoprotein	-
EHW96_RS12125	2690181..2691041	+	861	WP_004156324.1	acyl-CoA thioesterase II	-
EHW96_RS12130	2691116..2692417	-	1302	WP_004172214.1	ammonium transporter AmtB	-
EHW96_RS12135	2692435..2692773	-	339	WP_004156319.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T152431 WP_004156337.1 NZ_CP050258:2688541-2688759 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T152431 NZ_CP066151:881545-881952 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT152431 WP_013035913.1 NZ_CP050258:2688132-2688521 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT152431 NZ_CP066151:881298-881564 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0