Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 359629..360200 Replicon chromosome
Accession NZ_CP050255
Organism Enterococcus faecium strain DT1-1

Toxin (Protein)


Gene name mazF Uniprot ID R2Q2D5
Locus tag F0186_RS01715 Protein ID WP_002341043.1
Coordinates 359859..360200 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag F0186_RS01710 Protein ID WP_025477367.1
Coordinates 359629..359859 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0186_RS01690 355893..356486 + 594 WP_002342793.1 malonate decarboxylase holo-ACP synthase -
F0186_RS01695 356510..357595 + 1086 WP_002342794.1 acyltransferase family protein -
F0186_RS01700 357617..358486 + 870 WP_002342795.1 triphosphoribosyl-dephospho-CoA synthase MdcB -
F0186_RS01705 358598..359362 + 765 WP_002341045.1 TIM44-like domain-containing protein -
F0186_RS01710 359629..359859 + 231 WP_025477367.1 hypothetical protein Antitoxin
F0186_RS01715 359859..360200 + 342 WP_002341043.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F0186_RS01720 360380..360739 + 360 WP_156238084.1 hypothetical protein -
F0186_RS01725 360916..362997 + 2082 WP_002342796.1 BglG family transcription antiterminator -
F0186_RS01730 363033..363335 + 303 Protein_345 PTS sugar transporter subunit IIB -
F0186_RS01735 363361..364650 + 1290 WP_002341041.1 PTS sugar transporter subunit IIC -
F0186_RS01740 364669..365076 + 408 Protein_347 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13316.66 Da        Isoelectric Point: 9.9258

>T152423 WP_002341043.1 NZ_CP050255:359859-360200 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T152423 NZ_CP066149:c2058904-2058503 [Vibrio sp. ED004]
ATGGACATTATTTGTTTTCCATTCGAGCGAGTCATAGAAATCAATACATTCATACTCGATACTGAGCCTGGTATGAACGG
GGCCGTCGATATTCCTAAATTGCAAGGTGCTTTAGGTAGGATTGATAATGCCATTGTCTATGATGGTTTAGACGATATTT
TTGAGATTGCTGCAAAATATACGGCTAGCATTGCGGTAGCCCATGCGCTTCCGGATGCAAATAAACGAACTGGATTAATG
GTGGCTCTTGAGTACTTATCTCTAAACGACTATGAGTTGACCAGCGATAACGAGTTGTTGGCTGATGCCGTTCGAGACCT
AGTGCTCGCTGATATCTCGGAAACCGATTTCGCAGATATTTTGTATGCTCAATATGCGCAAGAAAACAACCTCGTTGATT
GA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT152423 WP_025477367.1 NZ_CP050255:359629-359859 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT152423 NZ_CP066149:c2059074-2058904 [Vibrio sp. ED004]
ATGAATAAGAAAATTGAATCTTATGGTGTGGGTGCGGTTGAAAGACCTAGAGTTAAAGCTACAAAAAAACTCGATTTGTC
AGGAACTTACGGACAACAAATTGTTAAATCAGAGTCCAAATTAGCATTGAGAACCCACAGAAAAACGTTTGAAAAACTGG
CTCATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829AD04

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References