Detailed information of TA system TA152422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 13592..14201
• Replicon: plasmid pIPMB12
• Accession: NZ_CP050254
• Organism: Orbus sp. IPMB12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IPMB12_RS12010
• Protein ID: WP_166917820.1
• Coordinates: 13893..14201 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IPMB12_RS12005
• Protein ID: WP_166917819.1
• Coordinates: 13592..13903 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPMB12_RS11980	8822..9574	-	753	WP_166917815.1	conjugal transfer protein TrbF	-
IPMB12_RS11985	9571..12147	-	2577	WP_166917816.1	VirB4 family type IV secretion/conjugal transfer ATPase	-
IPMB12_RS11990	12144..12455	-	312	WP_166917817.1	conjugal transfer protein TrbD	-
IPMB12_RS11995	12465..12878	-	414	WP_208293601.1	TrbC/VirB2 family protein	-
IPMB12_RS12000	13000..13557	+	558	WP_166917818.1	recombinase family protein	-
IPMB12_RS12005	13592..13903	+	312	WP_166917819.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
IPMB12_RS12010	13893..14201	+	309	WP_166917820.1	type II toxin-antitoxin system YafQ family toxin	Toxin
IPMB12_RS12015	14306..17248	-	2943	WP_208293600.1	DUF1738 domain-containing protein	-
IPMB12_RS12020	17241..17438	-	198	WP_166917822.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..32446	32446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (12-101)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (14-99)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11903.52 Da
• Isoelectric Point: 9.4385

>T152422 WP_166917820.1 NZ_CP050254:13893-14201 [Orbus sp. IPMB12]
VTNKKRANLPRRIEYTKEFSKSWNRYNKAGRRNMNDAIIIMQLLFANRTIPAEYNDHFLAGSEWDGAKELHIGGDFLLIY
RIDEKQNLITFVDIGSHSELFG

Nucleotide

• Length: 309 bp

>T152422 NZ_CP066149:c2052267-2051704 [Vibrio sp. ED004]
ATGCACTTGGCCAACACTGGCCTGTTTAGAGCGCGATGGACAGAACAAATACATGATGAATGGACTCGAAATCTACTGAA
AAATAGGCCAGAGCTGAATACAGAGCAACTGCAAAGAACTCGACAGTTAATGAACGCGAATGTTCCAGACTGTTTGATTG
AAGGGTATGAGCCTTTAATTGATGGCCTTACTTTACCCGATGTTAATGATAGACATGTTGTCGCTGCCGCTATTAAAGGT
CAGGCTGAGTCTATTATTACGTTTAACCTAAAAGACTTTCCTTCTAGCGAACTCGAACCTTTAGGTCTATCGGCAATACA
TCCAGACGAGTTCCTGTACGACATGTTCGAATTGGACAATAGCGCATGCGTTAAGGCAGCTCAGCAACAAAGAGCTTCAT
TAAAAAGACCACCAATGAGCCAAGACGAGTTCTTAGCTTACTTACAAAAACAGAGACTCCCAAGCTTCGTCTCACACTTA
AAGAGCTTCAAGCTTCAAGTTGATGCTTTAGATCTGATTTGTAGTAACAAGTGTCGTCGACTCGACAAAAATCAGCCAGC
ATAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11372.96 Da
• Isoelectric Point: 5.8244

>AT152422 WP_166917819.1 NZ_CP050254:13592-13903 [Orbus sp. IPMB12]
MCAQNDFFRGIYIMNATNINTRIDAELKADATNILGACGLTMSTAIRIFLEQVVQTQGLPFDVKRTPSAKTARALKEANE
LEKTGSHRFDSINGMVEDMTSDK

Nucleotide

• Length: 312 bp

>AT152422 NZ_CP066149:c2052785-2052333 [Vibrio sp. ED004]
ATGAATATTGCTACAAAATTGCCAAGCGCTGAAGAAATGGCACTGGCTAAGCTTGGTAGCCAAGAGTTGTCAGCCGTTAT
AGAAATTAATGGTGAGGCTCAAAGGATTAATGTTGTCGATAAGTCTGGTAAGACTCACGAAATCACTATTCCAGTAAGCG
CATTGAATATGATGATCGAGGTGCTGACTCAATTAGGTCAAGGCAACTCTGTTAGCATCACACCTATTCATGCGGAACTC
ACAACGCAAGAAGGTGCTGATATGCTAAGCATGTCTCGTCCTACTTTCATTAAGCTTCTGGATTCTGGTGATATTCCGTT
TAGTCGTACTGGCAACCGTAGAAAGGTAGCTTATGCTGATCTTATGGAGTACAAGCACCGCCTAGAAGAGAACAAACTAA
CTGCTCTTGCTGAACTATCAGCATTAGACCAAGAAATGGATATGGGATATTAA