Detailed information of TA system TA152421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1942506..1943440
• Replicon: chromosome
• Accession: NZ_CP050253
• Organism: Orbus sp. IPMB12

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IPMB12_RS08795
• Protein ID: WP_166916912.1
• Coordinates: 1942979..1943440 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IPMB12_RS08790
• Protein ID: WP_166916911.1
• Coordinates: 1942506..1942982 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPMB12_RS08780	1939076..1940674	+	1599	WP_166916909.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
IPMB12_RS08785	1940688..1942112	+	1425	WP_166916910.1	dihydrolipoyl dehydrogenase	-
IPMB12_RS08790	1942506..1942982	+	477	WP_166916911.1	DUF2384 domain-containing protein	Antitoxin
IPMB12_RS08795	1942979..1943440	+	462	WP_166916912.1	RES domain-containing protein	Toxin
IPMB12_RS08800	1943746..1944489	+	744	WP_166916913.1	DUF1471 domain-containing protein	-
IPMB12_RS08805	1944648..1945184	+	537	WP_166916914.1	hypothetical protein	-
IPMB12_RS08810	1945486..1946319	+	834	WP_166916915.1	hypothetical protein	-
IPMB12_RS08815	1946335..1946922	-	588	WP_166916916.1	serine acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-139)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (38-100)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 17248.94 Da
• Isoelectric Point: 8.3956

>T152421 WP_166916912.1 NZ_CP050253:1942979-1943440 [Orbus sp. IPMB12]
VKLYRIVKTEHIASAWSGYGAQQFGGRWNHPGRAMVYTASSISLAMLEILVHLQKSQFMSSYSLLSIEIPDKKLLNLDSD
KLSRKWNTYPALDETMDVGDKWLEEGKSVGLIVPSVIIPLEKNVVLNPAHPDFSGYIKEQTIQLVSFDSRLIK

Nucleotide

• Length: 462 bp

>T152421 NZ_CP066146:c8614-8333 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATTA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17569.99 Da
• Isoelectric Point: 6.4879

>AT152421 WP_166916911.1 NZ_CP050253:1942506-1942982 [Orbus sp. IPMB12]
MTNKVKEYRKTAEPIASSYHSDKIWQLAGFPASGGLTLIQELQRGLSVDVINRLNNGLNLSKKYVLEITGINERNLARRK
KTDDQRLNPEESERVARLVRVMDIAIDLFDGDKDAAIDWLNTPAIALGNRSPVELLATETGALEVSNLIGRLEHGVFS

Nucleotide

• Length: 477 bp

>AT152421 NZ_CP066146:c8855-8604 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA