Detailed information of TA system TA152420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 84244..84941
• Replicon: plasmid pStJH
• Accession: NZ_CP050250
• Organism: Sphingopyxis terrae subsp. terrae strain YC-JH3

Toxin (Protein)

• Gene name: tad
• Uniprot ID: T0J3S2
• Locus tag: HBA51_RS18515
• Protein ID: WP_013041565.1
• Coordinates: 84244..84618 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: T0G937
• Locus tag: HBA51_RS18520
• Protein ID: WP_006964370.1
• Coordinates: 84615..84941 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA51_RS18500 (HBA51_18510)	80611..81375	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
HBA51_RS18505 (HBA51_18515)	81438..83549	+	2112	Protein_81	Tn3 family transposase	-
HBA51_RS18510 (HBA51_18520)	83568..83822	-	255	WP_013041563.1	hypothetical protein	-
HBA51_RS18515 (HBA51_18525)	84244..84618	+	375	WP_013041565.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBA51_RS18520 (HBA51_18530)	84615..84941	+	327	WP_006964370.1	helix-turn-helix transcriptional regulator	Antitoxin
HBA51_RS18525 (HBA51_18535)	84941..85183	+	243	WP_013041566.1	hypothetical protein	-
HBA51_RS18530 (HBA51_18540)	85176..85475	+	300	WP_013041567.1	hypothetical protein	-
HBA51_RS18535 (HBA51_18545)	85496..86608	-	1113	WP_013041568.1	muramidase	-
HBA51_RS19300	86611..86856	-	246	WP_239659586.1	hypothetical protein	-
HBA51_RS18540 (HBA51_18550)	86865..88325	-	1461	WP_254536015.1	P-type conjugative transfer protein TrbL	-
HBA51_RS18545 (HBA51_18555)	88336..88578	-	243	WP_019053956.1	EexN family lipoprotein	-
HBA51_RS18550 (HBA51_18560)	88588..89343	-	756	WP_022675885.1	P-type conjugative transfer protein TrbJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..197269	197269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (15-94)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13960.67 Da
• Isoelectric Point: 9.4885

>T152420 WP_013041565.1 NZ_CP050250:84244-84618 [Sphingopyxis terrae subsp. terrae]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA

Nucleotide

• Length: 375 bp

>T152420 NZ_CP066145:39977-40324 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11792.57 Da
• Isoelectric Point: 7.1681

>AT152420 WP_006964370.1 NZ_CP050250:84615-84941 [Sphingopyxis terrae subsp. terrae]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA

Nucleotide

• Length: 327 bp

>AT152420 NZ_CP066145:40324-40623 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J7XHG4

Antitoxin

• AlphaFold DB: A0A0J8A803