Detailed information of TA system TA152416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2688131..2688758
• Replicon: chromosome
• Accession: NZ_CP050244
• Organism: Erwinia amylovora strain TS3238

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: EHX00_RS12100
• Protein ID: WP_004156337.1
• Coordinates: 2688540..2688758 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EHX00_RS12095
• Protein ID: WP_013035913.1
• Coordinates: 2688131..2688520 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHX00_RS12075	2683150..2686296	+	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit	-
EHX00_RS12080	2686444..2686725	+	282	WP_004156350.1	type B 50S ribosomal protein L31	-
EHX00_RS12085	2686713..2686859	+	147	WP_004156349.1	type B 50S ribosomal protein L36	-
EHX00_RS12090	2687631..2687984	+	354	WP_004156342.1	hypothetical protein	-
EHX00_RS12095	2688131..2688520	+	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
EHX00_RS12100	2688540..2688758	+	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
EHX00_RS12110	2689058..2689375	+	318	WP_004156334.1	MGMT family protein	-
EHX00_RS12115	2689398..2689955	-	558	WP_004156333.1	YbaY family lipoprotein	-
EHX00_RS12120	2690180..2691040	+	861	WP_004156324.1	acyl-CoA thioesterase II	-
EHX00_RS12125	2691115..2692416	-	1302	WP_004172214.1	ammonium transporter AmtB	-
EHX00_RS12130	2692434..2692772	-	339	WP_004156319.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T152416 WP_004156337.1 NZ_CP050244:2688540-2688758 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T152416 NZ_CP066144:4386945-4387202 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT152416 WP_013035913.1 NZ_CP050244:2688131-2688520 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT152416 NZ_CP066144:4387214-4387765 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0