Detailed information of TA system TA152396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2687659..2688286
• Replicon: chromosome
• Accession: NZ_CP050240
• Organism: Erwinia amylovora strain FB-20

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: EGZ89_RS12110
• Protein ID: WP_004156337.1
• Coordinates: 2688068..2688286 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EGZ89_RS12105
• Protein ID: WP_013035913.1
• Coordinates: 2687659..2688048 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGZ89_RS12085	2682678..2685824	+	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit	-
EGZ89_RS12090	2685972..2686253	+	282	WP_004156350.1	type B 50S ribosomal protein L31	-
EGZ89_RS12095	2686241..2686387	+	147	WP_004156349.1	type B 50S ribosomal protein L36	-
EGZ89_RS12100	2687159..2687512	+	354	WP_004156342.1	hypothetical protein	-
EGZ89_RS12105	2687659..2688048	+	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
EGZ89_RS12110	2688068..2688286	+	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
EGZ89_RS12120	2688586..2688903	+	318	WP_004156334.1	MGMT family protein	-
EGZ89_RS12125	2688926..2689483	-	558	WP_004156333.1	YbaY family lipoprotein	-
EGZ89_RS12130	2689708..2690568	+	861	WP_004156324.1	acyl-CoA thioesterase II	-
EGZ89_RS12135	2690643..2691944	-	1302	WP_004172214.1	ammonium transporter AmtB	-
EGZ89_RS12140	2691962..2692300	-	339	WP_004156319.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T152396 WP_004156337.1 NZ_CP050240:2688068-2688286 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T152396 NZ_CP066144:c47349-46924 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT152396 WP_013035913.1 NZ_CP050240:2687659-2688048 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT152396 NZ_CP066144:c47715-47380 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0