Detailed information of TA system TA152389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 10517..11073
• Replicon: plasmid pAsmB
• Accession: NZ_CP050189
• Organism: Aeromonas salmonicida subsp. masoucida strain RZ6S-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2H9U809
• Locus tag: G9457_RS24065
• Protein ID: WP_042468953.1
• Coordinates: 10517..10792 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G9457_RS24070
• Protein ID: WP_174416604.1
• Coordinates: 10792..11073 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9457_RS24040 (G9457_24135)	6252..6458	-	207	WP_258312897.1	YcaO-like family protein	-
G9457_RS24045 (G9457_24140)	6439..7451	-	1013	Protein_9	IS630 family transposase	-
G9457_RS24050 (G9457_24145)	7663..8580	-	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
G9457_RS24055 (G9457_24150)	8629..8814	-	186	WP_088814096.1	hypothetical protein	-
G9457_RS24060 (G9457_24155)	8816..9730	-	915	WP_042468951.1	TOMM precursor leader peptide-binding protein	-
G9457_RS24065 (G9457_24160)	10517..10792	-	276	WP_042468953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G9457_RS24070 (G9457_24165)	10792..11073	-	282	WP_174416604.1	ribbon-helix-helix domain-containing protein	Antitoxin
G9457_RS24075 (G9457_24170)	11285..11845	+	561	WP_042468955.1	recombinase family protein	-
G9457_RS24080 (G9457_24175)	11849..14875	+	3027	Protein_16	Tn3 family transposase	-
G9457_RS24085 (G9457_24180)	14910..15584	+	675	Protein_17	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	aopO / aopO / aopO / aopO / sycO	1..33232	33232
	-	inside	IScluster/Tn	-	aopO / aopO / aopO	329..22400	22071

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10685.43 Da
• Isoelectric Point: 9.5291

>T152389 WP_042468953.1 NZ_CP050189:c10792-10517 [Aeromonas salmonicida subsp. masoucida]
MELKWTSKALSDLARLYEFLALANKPAAARAVQALTQAPTILLTNPRIGEQLFQFEPREVRRILVGEYEVRYEILDSIIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T152389 NZ_CP066143:c3701372-3700962 [Klebsiella pneumoniae U-0608239]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10351.80 Da
• Isoelectric Point: 5.6624

>AT152389 WP_174416604.1 NZ_CP050189:c11073-10792 [Aeromonas salmonicida subsp. masoucida]
MKGNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWLAQEEDRDRLTQEALADVDAGRVIDHLAVQAWA
DSLNTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT152389 NZ_CP066143:c3701619-3701353 [Klebsiella pneumoniae U-0608239]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2H9U809