Detailed information of TA system TA152386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 497172..497812
• Replicon: chromosome
• Accession: NZ_CP050187
• Organism: Aeromonas salmonicida subsp. masoucida strain RZ6S-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A807Z4U5
• Locus tag: G9457_RS02460
• Protein ID: WP_005320982.1
• Coordinates: 497172..497585 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A807Z5T0
• Locus tag: G9457_RS02465
• Protein ID: WP_005320985.1
• Coordinates: 497582..497812 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9457_RS02435 (G9457_02450)	493545..494165	+	621	WP_232484104.1	subclass B2 metallo-beta-lactamase	-
G9457_RS02440 (G9457_02455)	494313..495230	+	918	WP_088814123.1	IS5-like element ISAs4 family transposase	-
G9457_RS02445 (G9457_02460)	495465..496382	+	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
G9457_RS02450 (G9457_02465)	496386..496706	-	321	WP_232479382.1	hypothetical protein	-
G9457_RS02455 (G9457_02470)	496795..497046	-	252	WP_258312852.1	hypothetical protein	-
G9457_RS02460 (G9457_02475)	497172..497585	-	414	WP_005320982.1	PIN domain-containing protein	Toxin
G9457_RS02465 (G9457_02480)	497582..497812	-	231	WP_005320985.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
G9457_RS02470 (G9457_02485)	497881..498186	-	306	WP_225854310.1	integrase	-
G9457_RS02475 (G9457_02490)	498180..498569	-	390	WP_139403308.1	site-specific integrase	-
G9457_RS02480 (G9457_02495)	498986..499903	-	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
G9457_RS02485 (G9457_02500)	499971..500372	+	402	Protein_469	IS4 family transposase	-
G9457_RS02490 (G9457_02505)	500445..501836	-	1392	WP_034283822.1	T3SS effector protein-tyrosine-phosphatase AopH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	aopH	494313..511461	17148
	-	inside	IScluster/Tn	-	aopH	492078..511461	19383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-42)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14935.23 Da
• Isoelectric Point: 5.7146

>T152386 WP_005320982.1 NZ_CP050187:c497585-497172 [Aeromonas salmonicida subsp. masoucida]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Nucleotide

• Length: 414 bp

>T152386 NZ_CP066143:c490364-490146 [Klebsiella pneumoniae U-0608239]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCAGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8699.88 Da
• Isoelectric Point: 4.7419

>AT152386 WP_005320985.1 NZ_CP050187:c497812-497582 [Aeromonas salmonicida subsp. masoucida]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Nucleotide

• Length: 231 bp

>AT152386 NZ_CP066143:c490765-490391 [Klebsiella pneumoniae U-0608239]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACTTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A807Z4U5

Antitoxin

• AlphaFold DB: A0A807Z5T0