Detailed information of TA system TA152385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2367224..2368202
• Replicon: chromosome
• Accession: NZ_CP050183
• Organism: Bacillus thuringiensis strain HER1410

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HBA75_RS12015
• Protein ID: WP_086412438.1
• Coordinates: 2367224..2367961 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: HBA75_RS12020
• Protein ID: WP_000237818.1
• Coordinates: 2368074..2368202 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA75_RS11990	2362839..2363246	+	408	WP_000072495.1	VOC family protein	-
HBA75_RS11995	2363268..2363648	+	381	WP_000497671.1	methyltransferase	-
HBA75_RS12000	2363806..2365491	-	1686	WP_033695152.1	alpha-keto acid decarboxylase family protein	-
HBA75_RS12005	2365599..2366081	+	483	WP_000191886.1	MarR family winged helix-turn-helix transcriptional regulator	-
HBA75_RS12010	2366248..2366985	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
HBA75_RS12015	2367224..2367961	+	738	WP_086412438.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HBA75_RS12020	2368074..2368202	+	129	WP_000237818.1	hypothetical protein	Antitoxin
HBA75_RS12025	2368275..2368451	+	177	WP_000852629.1	stage II sporulation protein SB	-
HBA75_RS12030	2368470..2368859	-	390	WP_016122519.1	YxeA family protein	-
HBA75_RS12035	2369070..2370539	+	1470	WP_049107769.1	beta-Ala-His dipeptidase	-
HBA75_RS12040	2370785..2371594	+	810	WP_000664544.1	C39 family peptidase	-
HBA75_RS12045	2371620..2372222	+	603	WP_000517259.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28263.02 Da
• Isoelectric Point: 9.0363

>T152385 WP_086412438.1 NZ_CP050183:2367224-2367961 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIAGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T152385 NZ_CP066142:3247586-3247789 [Morganella morganii]
ATGACTAAACTTGACTATCTCATGCGCTTACGTAAGTGCACTTCGATCGAAACACTGGAACGTGTCATTGAGAAGAACAA
ATATGAACTGACTGATGACGAGCTGGAGGTTTTTTATTCAGCTGCCGATCATCGTCTGGCCGAATTAACCATGAATAAAC
TGTATGACAAAATCCCGGCGTCTGTCTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT152385 WP_000237818.1 NZ_CP050183:2368074-2368202 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT152385 NZ_CP066142:3247164-3247523 [Morganella morganii]
ATGGACGAGTATTCTCCACGAAATTACGATATTGCGGTACTGAAGTATCTGTGTGATGAACTTTGCCGTGAAGGCACACA
AACTCTCATCAGAAGCAATAATTTTTGGGTTAATGATTTAGCCTCAGATTCAAACTTAAAACTTAACGAATTATTAGGTT
ACATTGCTGACGTGACCTGGAGTTTTAAGATAAAACATACACAAAATAAGGATCTCATCTCATTTGTCGATGAATACATC
GATGAAACTTACGCGCTGTTTGGTCAGATGGAAGTTTCATTTAATGATGTTGAGGAATGGAAGAAGATGGCATCACTGGT
AAGCAGCATGCTGACCAGCGAAGGGCATCTGGTCCACTAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5