Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 270331..270973 Replicon chromosome
Accession NZ_CP050183
Organism Bacillus thuringiensis strain HER1410

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag HBA75_RS01465 Protein ID WP_000635965.1
Coordinates 270623..270973 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HBA75_RS01460 Protein ID WP_000004570.1
Coordinates 270331..270618 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBA75_RS01435 265648..266610 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
HBA75_RS01440 266603..267175 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
HBA75_RS01445 267269..267628 + 360 WP_000583415.1 holo-ACP synthase -
HBA75_RS01450 267785..268735 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
HBA75_RS01455 268853..270022 + 1170 WP_000390615.1 alanine racemase -
HBA75_RS01460 270331..270618 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HBA75_RS01465 270623..270973 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HBA75_RS01470 271041..273209 + 2169 WP_140159320.1 RNA-binding transcriptional accessory protein -
HBA75_RS01475 273267..273383 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
HBA75_RS01480 273573..273902 + 330 WP_140159321.1 SprT family protein -
HBA75_RS01485 273921..275357 + 1437 WP_062804561.1 IS4 family transposase -
HBA75_RS01490 275487..275708 + 222 Protein_258 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 273921..275357 1436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T152384 WP_000635965.1 NZ_CP050183:270623-270973 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T152384 NZ_CP066142:2135712-2136056 [Morganella morganii]
GTGTGGACAGTATTATTAACGGATTATTTTAATGAATGGTTGTCAAAACAAGATACCCGGATGAAAGAAAAAGTGCTCGC
ATCTCTTGGCAATCTTGAAATTTATGGCCCTGTTCTGACAAGACCTTATGTTGATACAGTTAAAACTTCTCAGTACCCGA
ATATGAAAGAATTGAGAATTTGGCATGCTGGTCGTGCAATACGTGCATTTTTTGCCTTTGATCCTGAAAGGCAGGCCATT
ATTTTATGTGCCGGAGATAAAGGGAAGGATAAACGATTTTATCAGACGATGATTCGTATTGCGGACGAACAATTTTCCTC
ATATCTTTCAGCAACGCAGGAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT152384 WP_000004570.1 NZ_CP050183:270331-270618 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT152384 NZ_CP066142:2136061-2136360 [Morganella morganii]
ATGAAAACATTACAGGAAGTGATAGCGGCTCAATCACCGGAGAGCCGTGAGCGGATAAAACAACTGACTGATGAGCTGGT
TCTTGAAACCGGACTGCAACTTCTCAGAGAAGAACTTCATATCTCTCAGAAAACGCTGGCAAATAATCTTGGTATATCAC
AGCCGGCTGTTACTCAGATTGAGCAGCGCGGTAATGATATCAAACTGGCAACGCTGAAACGCTATATTGAGGCAATGGGC
GGAACACTTCGTCTGACGATTGAACTACCGACCGGTGATAACCGGATTTTTCGTGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References