Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 1848430..1849938 Replicon chromosome
Accession NZ_CP050149
Organism Pseudomonas aeruginosa strain CHA

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag HBA22_RS08615 Protein ID WP_003119682.1
Coordinates 1848430..1849017 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A5B3ABU1
Locus tag HBA22_RS08620 Protein ID WP_003119683.1
Coordinates 1849084..1849938 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBA22_RS08610 (HBA22_08595) 1844685..1848164 - 3480 WP_003117605.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -
HBA22_RS08615 (HBA22_08600) 1848430..1849017 + 588 WP_003119682.1 GNAT family protein Toxin
HBA22_RS08620 (HBA22_08605) 1849084..1849938 + 855 WP_003119683.1 AraC family transcriptional regulator Antitoxin
HBA22_RS08625 (HBA22_08610) 1850035..1852200 + 2166 WP_003122442.1 TonB-dependent receptor family protein -
HBA22_RS08630 (HBA22_08615) 1852375..1854249 + 1875 WP_003122443.1 MFS transporter -
HBA22_RS08635 (HBA22_08620) 1854310..1854519 - 210 WP_003115568.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T152306 WP_003119682.1 NZ_CP050149:1848430-1849017 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T152306 NZ_CP066108:66872-67264 [Enterobacter hormaechei]
ATGCTGCATATGCTGGACACTAACATCGTAAGCCATCTGGTGAGGCAGCATCCTGAAGTAGTAAACCGATATTCGCAAAT
TACGCCTGAAAAGATGTGTATTTCAAGCGTAACGGAAGCGGAATTGCTTTACGGCGTAGCCAAAAAGCAAAACAACAAGC
TGCACGAAACCATAATGGAATTTCTTAAAACCATCACTATCTGCGCCTGGGATAGCGAAGCCGCAGCCACCTACGGTGAA
CTGCGTGCGGCAATGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAGTTGATTGCAGCACATGCTATCAGCCGGGG
CACGACGATTGTCACGAACGATCATGCGTTCGGGATGGTACAGGATCTTACGGTTGAGGACTGGACTCAATAA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31875.19 Da        Isoelectric Point: 7.5295

>AT152306 WP_003119683.1 NZ_CP050149:1849084-1849938 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Download         Length: 855 bp

>AT152306 NZ_CP066108:66608-66868 [Enterobacter hormaechei]
ATGGAACGCACAGCGAAACTATTTAAAAAAGGACGAAACCAGGCCGTGGTCTTGCCTGCGGAATTTGCATTTGAAACGGA
GAGTGTTTATATCCGCCGGGATGACGAGGGGAATGTGGTTTTGACGGCAAGGTCCGAGAAAGAACGACACAGAGATAACT
TTTTGCGCATGCTAAGACAAACGCATGTGCCTGATACCTTTCTAAGTAAAGAGGAGCGCAATCAGAGCTATACGACAAGA
AATCCTCTGGAAGGGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB A0A5B3ABU1

References