Detailed information of TA system TA152296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4692834..4694092
• Replicon: chromosome
• Accession: NZ_CP050147
• Organism: Pseudomonas aeruginosa strain A5803

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: HBA20_RS21960
• Protein ID: WP_003086331.1
• Coordinates: 4692834..4693589 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HBA20_RS21965
• Protein ID: WP_003086329.1
• Coordinates: 4693724..4694092 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA20_RS21950 (HBA20_21980)	4688006..4690549	+	2544	WP_003134033.1	acyl-homoserine-lactone acylase QuiP	-
HBA20_RS21955 (HBA20_21985)	4690704..4692065	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
HBA20_RS21960 (HBA20_21990)	4692834..4693589	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
HBA20_RS21965 (HBA20_21995)	4693724..4694092	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HBA20_RS21970 (HBA20_22000)	4694165..4695451	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
HBA20_RS21975 (HBA20_22005)	4695507..4697000	-	1494	WP_033954387.1	aldehyde dehydrogenase family protein	-
HBA20_RS21980 (HBA20_22010)	4697174..4697650	+	477	WP_003086322.1	hypothetical protein	-
HBA20_RS21985	4697653..4697928	-	276	WP_171410771.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T152296 WP_003086331.1 NZ_CP050147:c4693589-4692834 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T152296 NZ_CP066101:c1117740-1117522 [Enterobacter hormaechei]
ATGTCTGAAAAACCATTAACGAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGCCTTGCTGAAC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT152296 WP_003086329.1 NZ_CP050147:c4694092-4693724 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT152296 NZ_CP066101:c1118142-1117768 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAATCATGGCTGGGTAAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATTGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCAAACCCGGTTAGTCTTTCCTGTTAG

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7