Detailed information of TA system TA152295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3743053..3744273
• Replicon: chromosome
• Accession: NZ_CP050147
• Organism: Pseudomonas aeruginosa strain A5803

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: HBA20_RS17510
• Protein ID: WP_003110488.1
• Coordinates: 3743695..3744273 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HBA20_RS17505
• Protein ID: WP_003088112.1
• Coordinates: 3743053..3743607 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA20_RS17480 (HBA20_17515)	3738621..3739172	+	552	WP_003139878.1	XRE family transcriptional regulator	-
HBA20_RS17485 (HBA20_17520)	3739169..3739567	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HBA20_RS17490 (HBA20_17525)	3739580..3739903	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HBA20_RS17495 (HBA20_17530)	3740234..3740695	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HBA20_RS17500 (HBA20_17535)	3740700..3742895	+	2196	WP_023875495.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HBA20_RS17505 (HBA20_17540)	3743053..3743607	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
HBA20_RS17510 (HBA20_17545)	3743695..3744273	+	579	WP_003110488.1	HD domain-containing protein	Toxin
HBA20_RS17515 (HBA20_17550)	3744382..3745569	-	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HBA20_RS17520 (HBA20_17555)	3745559..3747730	-	2172	WP_023106617.1	type I secretion system permease/ATPase	-
HBA20_RS17525 (HBA20_17560)	3747720..3748997	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T152295 WP_003110488.1 NZ_CP050147:3743695-3744273 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T152295 NZ_CP066101:c936899-936558 [Enterobacter hormaechei]
ATGAAACCTCTACCTGCAACAACTTCGCGGGCGGCGAAGCCTTGTCTGTCGCCCGTTGCAGTCTGGCAAATACTGCTTAC
ACACCTGCTGGAGCAGCACTATGGTCTGACGCTAAACGATACGCCGTTCAGTGATGAAACCGTTATTAAGGAACATATTG
ATGCCGGGATCACCCTGGCCGATGCCGTTAATTTTCTGGTGGAGAAATACGAGCTGGTGCGTATAGATCGCAGGGGATTT
AGCAGGCAGGAACAATCGCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGTAACTGGCCTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT152295 WP_003088112.1 NZ_CP050147:3743053-3743607 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT152295 NZ_CP066101:c937237-936920 [Enterobacter hormaechei]
ATGAGCAATATCACATGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGATAGGAACCGGAT
GCATTATCTGGCTGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAATGCCTGAAGTTGGATGTCGCATTCCCGC
ATTTTATCAGTCAGATGGAATCAATGCTGACCACCGGTGAACTGAATCCCCGCCATCCACACTGCATCGCCCTGTATCAC
AACGGTTTTACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCTGTTTATCCCAGCCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4