Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 10004..10674 Replicon plasmid pENS2
Accession NZ_CP050141
Organism Komagataeibacter rhaeticus strain ENS 9a1a

Toxin (Protein)


Gene name vapC Uniprot ID A0A7H4IM06
Locus tag GWK63_RS16900 Protein ID WP_007396297.1
Coordinates 10252..10674 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0N1N5A1
Locus tag GWK63_RS16895 Protein ID WP_039733231.1
Coordinates 10004..10255 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWK63_RS16870 5024..5290 - 267 WP_053323887.1 conjugal transfer protein TraD -
GWK63_RS16875 5355..5714 - 360 WP_191011408.1 conjugal transfer protein TraD -
GWK63_RS16880 5811..9188 + 3378 WP_166498088.1 Ti-type conjugative transfer relaxase TraA -
GWK63_RS16885 9235..9657 + 423 WP_007396294.1 putative toxin-antitoxin system toxin component, PIN family -
GWK63_RS16890 9654..9917 + 264 WP_007396295.1 toxin-antitoxin system HicB family antitoxin -
GWK63_RS16895 10004..10255 + 252 WP_039733231.1 plasmid stabilization protein Antitoxin
GWK63_RS16900 10252..10674 + 423 WP_007396297.1 type II toxin-antitoxin system VapC family toxin Toxin
GWK63_RS16905 10739..10933 + 195 WP_010512461.1 type II toxin-antitoxin system VapB family antitoxin -
GWK63_RS16910 10930..11322 + 393 WP_007396299.1 PIN domain nuclease -
GWK63_RS16915 11615..12283 + 669 WP_025439951.1 hypothetical protein -
GWK63_RS16920 12598..13299 + 702 Protein_19 Fic family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..13375 13375


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15283.53 Da        Isoelectric Point: 5.2273

>T152291 WP_007396297.1 NZ_CP050141:10252-10674 [Komagataeibacter rhaeticus]
MILLDTNVISEPWKPVPEQRVLAWIDGQAIETLFLSAVTVAELRFGIGAMPSGRRRSVLHERLEQEVLPLFAGRVLSFDL
AASQAYAELMVRARSEGRAIGKADGYIAATAASRGYVVASRDVSPFEAAGVRVLNPWEDT

Download         Length: 423 bp

>T152291 NZ_CP066094:c5028230-5027859 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGAGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9079.36 Da        Isoelectric Point: 6.4584

>AT152291 WP_039733231.1 NZ_CP050141:10004-10255 [Komagataeibacter rhaeticus]
MSSVVIRNLPEETHRALKAQAMLHGRSTEAEIRTILEAAVRPPQRVRLGSLLASIGREAGVCDQDIAALQSRDQTPAEPM
SFE

Download         Length: 252 bp

>AT152291 NZ_CP066094:c5028475-5028233 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4IM06

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1N5A1

References