Detailed information of TA system TA152288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3333539..3334145
• Replicon: chromosome
• Accession: NZ_CP050139
• Organism: Komagataeibacter rhaeticus strain ENS 9a1a

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D6QDX8
• Locus tag: GWK63_RS15375
• Protein ID: WP_019087453.1
• Coordinates: 3333966..3334145 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A181CEU7
• Locus tag: GWK63_RS15370
• Protein ID: WP_061275760.1
• Coordinates: 3333539..3333955 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK63_RS15335	3329074..3330267	-	1194	WP_025439978.1	Fic family protein	-
GWK63_RS15340	3330728..3331231	+	504	WP_112209709.1	MucR family transcriptional regulator	-
GWK63_RS15345	3331261..3331776	-	516	WP_112209721.1	hypothetical protein	-
GWK63_RS15350	3331682..3331999	-	318	WP_158551941.1	hypothetical protein	-
GWK63_RS15355	3332349..3332771	-	423	WP_112209708.1	type II toxin-antitoxin system VapC family toxin	-
GWK63_RS15360	3332768..3333025	-	258	WP_112209707.1	plasmid stabilization protein	-
GWK63_RS15365	3333208..3333462	-	255	WP_112209706.1	hypothetical protein	-
GWK63_RS15370	3333539..3333955	-	417	WP_061275760.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GWK63_RS15375	3333966..3334145	-	180	WP_019087453.1	type II toxin-antitoxin system HicA family toxin	Toxin
GWK63_RS15385	3335878..3336300	-	423	WP_112209712.1	hypothetical protein	-
GWK63_RS15390	3336297..3336492	-	196	Protein_3020	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GWK63_RS15395	3337365..3338315	-	951	WP_112209711.1	replication initiator protein A	-
GWK63_RS15400	3338312..3338632	-	321	WP_202795770.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3183466..3439080	255614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-125)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6479.65 Da
• Isoelectric Point: 11.4111

>T152288 WP_019087453.1 NZ_CP050139:c3334145-3333966 [Komagataeibacter rhaeticus]
MKSADLIRELEKAGWKLDRVRGSHNVFKHPSRPGIVVVPHPKKDLGTGLVAAIRKQAGL

Nucleotide

• Length: 180 bp

>T152288 NZ_CP066094:c3236079-3235726 [Enterobacter hormaechei]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14827.67 Da
• Isoelectric Point: 4.3147

>AT152288 WP_061275760.1 NZ_CP050139:c3333955-3333539 [Komagataeibacter rhaeticus]
MRYPVVIERGSEATAFGVVFPDLPGCFSAGDTLDEAISNAEEAAAAWIDATLDAGEAVPSPSTLDAIQNNPDYEGWTVGF
VTIDPAVLDDRVERANISLPRRVLERLDALARSSHESRSGMIAAMTLQARLKPVVRSK

Nucleotide

• Length: 417 bp

>AT152288 NZ_CP066094:c3235721-3235419 [Enterobacter hormaechei]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A0D6QDX8

Antitoxin

• AlphaFold DB: A0A181CEU7