Detailed information of TA system TA152285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 410544..411225
• Replicon: chromosome
• Accession: NZ_CP050139
• Organism: Komagataeibacter rhaeticus strain ENS 9a1a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7H4IM06
• Locus tag: GWK63_RS01990
• Protein ID: WP_007396297.1
• Coordinates: 410544..410966 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W5YD93
• Locus tag: GWK63_RS01995
• Protein ID: WP_010512461.1
• Coordinates: 411031..411225 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK63_RS01965	405647..406006	-	360	WP_191011408.1	conjugal transfer protein TraD	-
GWK63_RS01970	406103..409480	+	3378	WP_166498088.1	Ti-type conjugative transfer relaxase TraA	-
GWK63_RS01975	409527..409949	+	423	WP_007396294.1	putative toxin-antitoxin system toxin component, PIN family	-
GWK63_RS01980	409946..410209	+	264	WP_007396295.1	toxin-antitoxin system HicB family antitoxin	-
GWK63_RS01985	410296..410547	+	252	WP_039733231.1	plasmid stabilization protein	-
GWK63_RS01990	410544..410966	+	423	WP_007396297.1	type II toxin-antitoxin system VapC family toxin	Toxin
GWK63_RS01995	411031..411225	+	195	WP_010512461.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GWK63_RS02000	411222..411614	+	393	WP_007396299.1	PIN domain nuclease	-
GWK63_RS02005	411907..412575	+	669	WP_025439951.1	hypothetical protein	-
GWK63_RS02010	412890..413591	+	702	Protein_392	Fic family protein	-
GWK63_RS02015	413761..414189	+	429	Protein_393	transposase	-
GWK63_RS02020	414191..414625	+	435	Protein_394	IS110 family transposase	-
GWK63_RS02025	414622..415674	-	1053	WP_039998278.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	375122..508835	133713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-47)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15283.53 Da
• Isoelectric Point: 5.2273

>T152285 WP_007396297.1 NZ_CP050139:410544-410966 [Komagataeibacter rhaeticus]
MILLDTNVISEPWKPVPEQRVLAWIDGQAIETLFLSAVTVAELRFGIGAMPSGRRRSVLHERLEQEVLPLFAGRVLSFDL
AASQAYAELMVRARSEGRAIGKADGYIAATAASRGYVVASRDVSPFEAAGVRVLNPWEDT

Nucleotide

• Length: 423 bp

>T152285 NZ_CP066094:c2605511-2605026 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAGCGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGAGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7268.49 Da
• Isoelectric Point: 10.4776

>AT152285 WP_010512461.1 NZ_CP050139:411031-411225 [Komagataeibacter rhaeticus]
MRTNIIIDDALMTDALKASGVKTKKEAVELGLRTLIRLKQQRELRTLRGKLDWQGDLDAMRSDA

Nucleotide

• Length: 195 bp

>AT152285 NZ_CP066094:c2605765-2605499 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7H4IM06

Antitoxin

• AlphaFold DB: A0A318QDR0