Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 410296..410966 Replicon chromosome
Accession NZ_CP050139
Organism Komagataeibacter rhaeticus strain ENS 9a1a

Toxin (Protein)


Gene name vapC Uniprot ID A0A7H4IM06
Locus tag GWK63_RS01990 Protein ID WP_007396297.1
Coordinates 410544..410966 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0N1N5A1
Locus tag GWK63_RS01985 Protein ID WP_039733231.1
Coordinates 410296..410547 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWK63_RS01960 405316..405582 - 267 WP_053323887.1 conjugal transfer protein TraD -
GWK63_RS01965 405647..406006 - 360 WP_191011408.1 conjugal transfer protein TraD -
GWK63_RS01970 406103..409480 + 3378 WP_166498088.1 Ti-type conjugative transfer relaxase TraA -
GWK63_RS01975 409527..409949 + 423 WP_007396294.1 putative toxin-antitoxin system toxin component, PIN family -
GWK63_RS01980 409946..410209 + 264 WP_007396295.1 toxin-antitoxin system HicB family antitoxin -
GWK63_RS01985 410296..410547 + 252 WP_039733231.1 plasmid stabilization protein Antitoxin
GWK63_RS01990 410544..410966 + 423 WP_007396297.1 type II toxin-antitoxin system VapC family toxin Toxin
GWK63_RS01995 411031..411225 + 195 WP_010512461.1 type II toxin-antitoxin system VapB family antitoxin -
GWK63_RS02000 411222..411614 + 393 WP_007396299.1 PIN domain nuclease -
GWK63_RS02005 411907..412575 + 669 WP_025439951.1 hypothetical protein -
GWK63_RS02010 412890..413591 + 702 Protein_392 Fic family protein -
GWK63_RS02015 413761..414189 + 429 Protein_393 transposase -
GWK63_RS02020 414191..414625 + 435 Protein_394 IS110 family transposase -
GWK63_RS02025 414622..415674 - 1053 WP_039998278.1 replication initiator protein A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 375122..508835 133713


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15283.53 Da        Isoelectric Point: 5.2273

>T152284 WP_007396297.1 NZ_CP050139:410544-410966 [Komagataeibacter rhaeticus]
MILLDTNVISEPWKPVPEQRVLAWIDGQAIETLFLSAVTVAELRFGIGAMPSGRRRSVLHERLEQEVLPLFAGRVLSFDL
AASQAYAELMVRARSEGRAIGKADGYIAATAASRGYVVASRDVSPFEAAGVRVLNPWEDT

Download         Length: 423 bp

>T152284 NZ_CP066094:c1382951-1382733 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9079.36 Da        Isoelectric Point: 6.4584

>AT152284 WP_039733231.1 NZ_CP050139:410296-410547 [Komagataeibacter rhaeticus]
MSSVVIRNLPEETHRALKAQAMLHGRSTEAEIRTILEAAVRPPQRVRLGSLLASIGREAGVCDQDIAALQSRDQTPAEPM
SFE

Download         Length: 252 bp

>AT152284 NZ_CP066094:c1383353-1382979 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4IM06

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1N5A1

References