Detailed information of TA system TA152276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84083..85706
• Replicon: plasmid unnamed
• Accession: NZ_CP050131
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain GSJ/2017-Sal-008

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HBA58_RS25020
• Protein ID: WP_140780865.1
• Coordinates: 84381..85706 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: HBA58_RS25015
• Protein ID: WP_024136327.1
• Coordinates: 84083..84376 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA58_RS24990	79955..81196	+	1242	WP_140780866.1	VWA domain-containing protein	-
HBA58_RS24995	81759..82040	-	282	WP_000077926.1	hypothetical protein	-
HBA58_RS25000	82090..82281	-	192	WP_000398480.1	hypothetical protein	-
HBA58_RS25005	82373..82714	-	342	WP_001151575.1	hypothetical protein	-
HBA58_RS25010	83087..83479	+	393	WP_000341066.1	hypothetical protein	-
HBA58_RS25015	84083..84376	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
HBA58_RS25020	84381..85706	+	1326	WP_140780865.1	type II toxin-antitoxin system HipA family toxin	Toxin
HBA58_RS25025	85767..85973	+	207	WP_000134171.1	hypothetical protein	-
HBA58_RS25030	86075..86458	+	384	WP_140780864.1	hypothetical protein	-
HBA58_RS25035	86498..87313	+	816	WP_001572439.1	HNH endonuclease	-
HBA58_RS25040	87567..87992	+	426	WP_001043843.1	hypothetical protein	-
HBA58_RS25045	88446..88682	+	237	Protein_85	hypothetical protein	-
HBA58_RS25050	88737..89036	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / oqxB / oqxA / blaCTX-M-14 / mcr-1.1 / tet(A) / qnrS2 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR	-	1..298622	298622

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50291.28 Da
• Isoelectric Point: 6.1927

>T152276 WP_140780865.1 NZ_CP050131:84381-85706 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHVEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T152276 NZ_CP066089:c2688283-2687909 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGGTGAAGCGAAGAAGGCTCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATCGGCGTTTCACAGGATATTGCTGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTCATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTTGCGGGGATTCCGGGAGTCATTACGCCTTACCATTTATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT152276 WP_024136327.1 NZ_CP050131:84083-84376 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT152276 NZ_CP066089:c2688525-2688283 [Citrobacter koseri]
ATGATGGCTGGGATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAAGTTATCAAGCGGCTTGATGACCTCAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAACTTCGCGAGGAGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1