Detailed information of TA system TA152275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 124730..125251
• Replicon: plasmid plas1
• Accession: NZ_CP050125
• Organism: Rhodococcus erythropolis strain KB1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: T5HL71
• Locus tag: G9444_RS31270
• Protein ID: WP_021334703.1
• Coordinates: 124730..125035 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G9444_RS31275
• Protein ID: WP_030538123.1
• Coordinates: 125036..125251 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9444_RS31250	120095..121153	-	1059	WP_165639875.1	IS110 family transposase	-
G9444_RS31255	121464..121811	+	348	WP_021334700.1	DUF1016 family protein	-
G9444_RS31260	121937..122734	-	798	WP_021334701.1	hypothetical protein	-
G9444_RS31265	122734..124131	-	1398	WP_080656785.1	hypothetical protein	-
G9444_RS31270	124730..125035	-	306	WP_021334703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G9444_RS31275	125036..125251	-	216	WP_030538123.1	hypothetical protein	Antitoxin
G9444_RS31280	125615..126334	-	720	WP_021334705.1	hypothetical protein	-
G9444_RS31285	126676..127458	+	783	WP_021334706.1	DUF4145 domain-containing protein	-
G9444_RS31290	127596..128909	+	1314	WP_030538128.1	IS256 family transposase	-
G9444_RS31295	128948..129250	+	303	Protein_138	IS110 family transposase	-
G9444_RS31300	129171..129380	-	210	WP_166503019.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..242066	242066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 15030.03 Da
• Isoelectric Point: 5.5500

>T152275 WP_021334703.1 NZ_CP050125:c125035-124730 [Rhodococcus erythropolis]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 306 bp

>T152275 NZ_CP066089:c2648885-2648577 [Citrobacter koseri]
ATGCACGTCATTTCAAAAGAACCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATACGGTCGCTGTA
CCGCTTAATGCGTGAAACGGACTTCTCTTCTCCGACTGAGATGAGAACTCTGATACCGAGCCTGGACAATTTTAAGTACC
GGAACAAATGGTGGGTTCTGGATGTGGGAGGCAACAACTTGAGGGTTATCGCCTACATCAATTTTGTGAATAAACGCTTC
TATGTGAAGCACATCAGCACCCACGCTGAATATGACAAGTTGACCCGCTATTACAGGGAGAACAAATAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 10614.73 Da
• Isoelectric Point: 5.5500

>AT152275 WP_030538123.1 NZ_CP050125:c125251-125036 [Rhodococcus erythropolis]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 216 bp

>AT152275 NZ_CP066089:c2648577-2648158 [Citrobacter koseri]
ATGAATACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTCGCCGCTGTTCCTTTCCTGGGAGGCAGCTCTTC
AGAGAGCGATTACCGTGAAGCGCTGTCTCTGGTGGATTATCTGATCGAGAACGACGATGAGAACCCGCTCATTGACTTCC
TGGCGAGCAAAATTGCCGACTATGAGGATAACAGCGACCGTTTCGCTGAGTTCAATAAAGGTATCGCTGAAATGCCGGTT
GGCGTGGCGCTGCTACGTACTCTGATTGACCAGTACAAGCTGTCCTACTCAGACCTGAAAGAGGAGATCGGTTCAAAGTC
GCTGGTGAGTCAGATCTTATCTGGTCAGCGCTCTCTGACTATCGCTCACATTAAGGCACTCTCTACTCGCTTCGGTGTGA
AGCCAGAGTGGTTTCTTTAA

Structures

Toxin

• AlphaFold DB: T5HL71