Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 975044..975633 Replicon chromosome
Accession NZ_CP050116
Organism Deinococcus radiodurans strain BNK-50

Toxin (Protein)


Gene name mazF Uniprot ID Q9RX98
Locus tag HAV23_RS04965 Protein ID WP_010887062.1
Coordinates 975280..975633 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q9RX99
Locus tag HAV23_RS04960 Protein ID WP_010887061.1
Coordinates 975044..975286 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAV23_RS04925 970056..970475 + 420 WP_010887054.1 DinB family protein -
HAV23_RS04930 970468..970839 + 372 Protein_958 inorganic pyrophosphatase -
HAV23_RS04935 970836..971519 + 684 WP_010887056.1 SDR family oxidoreductase -
HAV23_RS04940 971540..972502 + 963 WP_034349507.1 NAD(P)/FAD-dependent oxidoreductase -
HAV23_RS04945 972525..972704 + 180 WP_034349506.1 hypothetical protein -
HAV23_RS04950 972706..973335 + 630 WP_027479568.1 HAD family hydrolase -
HAV23_RS04955 973383..974864 - 1482 Protein_963 carboxylesterase/lipase family protein -
HAV23_RS04960 975044..975286 + 243 WP_010887061.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HAV23_RS04965 975280..975633 + 354 WP_010887062.1 endoribonuclease MazF Toxin
HAV23_RS04970 975686..975910 + 225 WP_162177573.1 hypothetical protein -
HAV23_RS04975 975907..976320 + 414 WP_010887064.1 type II toxin-antitoxin system VapC family toxin -
HAV23_RS04980 976317..978784 + 2468 Protein_968 ATP-dependent helicase HrpB -
HAV23_RS04985 978965..979189 - 225 WP_162177572.1 hypothetical protein -
HAV23_RS04990 979345..980121 - 777 WP_010887067.1 trans-aconitate 2-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Antitoxin PemI-like

(1-80)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 12450.24 Da        Isoelectric Point: 7.3219

>T152269 WP_010887062.1 NZ_CP050116:975280-975633 [Deinococcus radiodurans]
MVSDYVPDAGHLVWLNFTPQAGHEQGGRRPALVLSPAAYNGVTGLMQACPVTSRAKGYPFEVTLPAHLGVSGVVLADHCR
SLDWRSRRAEQLAEAPADVLAEVRGKLGSLLGMSEKA

Download         Length: 354 bp

>T152269 NZ_CP066085:4472732-4472950 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTATGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8911.12 Da        Isoelectric Point: 4.3178

>AT152269 WP_010887061.1 NZ_CP050116:975044-975286 [Deinococcus radiodurans]
MTSQIQKWGNSLALRIPKALAQQVGLTQSSEVELLLQDGQIVIRPVPARQYDLAALLAEMTPENLHGETDWGALEGREEW

Download         Length: 243 bp

>AT152269 NZ_CP066085:4472331-4472705 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTATCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7D2M
PDB 7D2P
PDB 7DHP
PDB 7D2Q
PDB 7D28
PDB 7D2N

Antitoxin

Source ID Structure
PDB 7D2P
PDB 7D2Q
PDB 7D2N
AlphaFold DB Q9RX99

References