Detailed information of TA system TA152255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 993039..993619
• Replicon: plasmid pRL3b2
• Accession: NZ_CP050109
• Organism: Rhizobium leguminosarum bv. trifolii strain 3B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A444I7Z5
• Locus tag: HA459_RS28200
• Protein ID: WP_018245324.1
• Coordinates: 993039..993422 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Y3WNA0
• Locus tag: HA459_RS28205
• Protein ID: WP_018245323.1
• Coordinates: 993419..993619 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA459_RS28165	988488..988708	-	221	Protein_915	Gfo/Idh/MocA family oxidoreductase	-
HA459_RS28170	989123..990217	+	1095	WP_018245327.1	hypothetical protein	-
HA459_RS28175	990514..991566	-	1053	WP_130729681.1	ribosome small subunit-dependent GTPase A	-
HA459_RS28180	991601..991777	-	177	WP_018071841.1	hypothetical protein	-
HA459_RS28185	991954..992283	+	330	WP_018517229.1	hypothetical protein	-
HA459_RS28195	992602..992829	-	228	WP_085990123.1	CopG family transcriptional regulator	-
HA459_RS28200	993039..993422	-	384	WP_018245324.1	type II toxin-antitoxin system VapC family toxin	Toxin
HA459_RS28205	993419..993619	-	201	WP_018245323.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HA459_RS28210	993784..994023	-	240	WP_155256963.1	hypothetical protein	-
HA459_RS28215	994156..994473	+	318	Protein_924	glycerol-3-phosphate dehydrogenase	-
HA459_RS28220	994622..994753	-	132	Protein_925	transposase domain-containing protein	-
HA459_RS28225	994813..995445	+	633	WP_018444521.1	DUF433 domain-containing protein	-
HA459_RS28230	995442..995816	+	375	WP_018245318.1	DUF5615 family PIN-like protein	-
HA459_RS28235	995888..997468	-	1581	WP_018245317.1	ABC transporter substrate-binding protein	-
HA459_RS28240	997590..998375	+	786	WP_065275917.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1232616	1232616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14117.31 Da
• Isoelectric Point: 9.1808

>T152255 WP_018245324.1 NZ_CP050109:c993422-993039 [Rhizobium leguminosarum bv. trifolii]
VILADTSIWIDHFRHVDAELRTIIEDDRLLCHPAVVGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASILLDRRAALWTRDKRLQAAAKKAGASLHKPANARN

Nucleotide

• Length: 384 bp

>T152255 NZ_CP066085:c157882-157475 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACTCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATTCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7124.23 Da
• Isoelectric Point: 11.3760

>AT152255 WP_018245323.1 NZ_CP050109:c993619-993419 [Rhizobium leguminosarum bv. trifolii]
MRSTINLDDTLMERARSLTGTKETAAIVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT152255 NZ_CP066085:c158129-157863 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A444I7Z5

Antitoxin

• AlphaFold DB: A0A7Y3WNA0