Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 993040..993620 Replicon plasmid pRL4b2
Accession NZ_CP050104
Organism Rhizobium leguminosarum bv. trifolii strain 4B

Toxin (Protein)


Gene name vapC Uniprot ID A0A444I7Z5
Locus tag HA460_RS28235 Protein ID WP_018245324.1
Coordinates 993040..993423 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7Y3WNA0
Locus tag HA460_RS28240 Protein ID WP_018245323.1
Coordinates 993420..993620 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA460_RS28200 988489..988709 - 221 Protein_915 Gfo/Idh/MocA family oxidoreductase -
HA460_RS28205 989124..990218 + 1095 WP_018245327.1 hypothetical protein -
HA460_RS28210 990515..991567 - 1053 WP_130729681.1 ribosome small subunit-dependent GTPase A -
HA460_RS28215 991602..991778 - 177 WP_018071841.1 hypothetical protein -
HA460_RS28220 991955..992284 + 330 WP_018517229.1 hypothetical protein -
HA460_RS28230 992603..992830 - 228 WP_085990123.1 CopG family transcriptional regulator -
HA460_RS28235 993040..993423 - 384 WP_018245324.1 type II toxin-antitoxin system VapC family toxin Toxin
HA460_RS28240 993420..993620 - 201 WP_018245323.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HA460_RS28245 993785..994024 - 240 WP_155256963.1 hypothetical protein -
HA460_RS28250 994157..994474 + 318 Protein_924 glycerol-3-phosphate dehydrogenase -
HA460_RS28255 994623..994754 - 132 Protein_925 transposase domain-containing protein -
HA460_RS28260 994814..995446 + 633 WP_018444521.1 DUF433 domain-containing protein -
HA460_RS28265 995443..995817 + 375 WP_018245318.1 DUF5615 family PIN-like protein -
HA460_RS28270 995889..997469 - 1581 WP_018245317.1 ABC transporter substrate-binding protein -
HA460_RS28275 997591..998376 + 786 WP_065275917.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..1232616 1232616


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14117.31 Da        Isoelectric Point: 9.1808

>T152251 WP_018245324.1 NZ_CP050104:c993423-993040 [Rhizobium leguminosarum bv. trifolii]
VILADTSIWIDHFRHVDAELRTIIEDDRLLCHPAVVGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASILLDRRAALWTRDKRLQAAAKKAGASLHKPANARN

Download         Length: 384 bp

>T152251 NZ_CP066076:c2513807-2512905 [Paraburkholderia ginsengisoli]
ATGTCCGCAGTCCCGTCCGCTACCGCCGATCAACCCGCCACGCTCGAAGCACGCCTTGCGCGCTTTCCGGCGCGGCAGGT
CTCGCTGGCGTCGCAACGCACGCTGAGCTATCGCGAAGCCGGTAGCGCCGGCAACAGCGCTAACAGCGACGCACTGCCGC
TCGTGCTGTTGCACGGTATCGGATCGGGCGCGGCATCGTGGGTGCAGCAATTCGAAGGACTCGGCGCGAACCGCCGCGTG
CTCGCGTGGGATGCGCCGGGTTATGGCGCGTCCACGCCGGTTGCGGCTGAATCGCCGGTTGCCGTCGACTACGCAGGTGT
ACTGAAGGAGTGGCTCGACGCGCTCGGCCTCGAACGTTGCGTTCTGGTCGGGCATTCGCTCGGCGCAATCATCGCCGGTG
CGTTTGCCGCCGCTCATCCGCAATTTGTGGCCGGTTTGCTGCTGCTGTCGCCTGCGGGCGGTTACGGCGCGGCATCGGCG
GAAGTGCGCGAGACCAAACGCGACCAACGCCTCGCGATGCTGAACGAACTCGGTCCGCAAGGTCTCGCCGAAAAACGCAG
CGCGAACATGCTGTCCGCTCACGCGAACGACGAAGCCCGCGCATGGGTGCGCTGGAACATGTCGCGCGTGATCCCGCACG
GCTACGCGCAAGCGACGCATCTGCTCGCCAACGCCGATCTCGCCGCCGACCTCTCGCGCTTCAAAGGCCGCATCAACGTC
GCGGTCGGCGCCGACGACACCATCACCCCTCCCGCGGCCTGCGAGCGAATCGCGCTCGCCGCGGGCACGAAATTGCAGGT
TGTGCCGCACGCGGGGCACGCCGGCTACATCGAAGCACCCGCCGCGTACACCGCGATCATCGACACGTTCTGCCGCGCGA
GCGGCGGACAACGGAGCCAATGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7124.23 Da        Isoelectric Point: 11.3760

>AT152251 WP_018245323.1 NZ_CP050104:c993620-993420 [Rhizobium leguminosarum bv. trifolii]
MRSTINLDDTLMERARSLTGTKETAAIVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT152251 NZ_CP066076:c2512904-2512017 [Paraburkholderia ginsengisoli]
ATGACGCCCGACACTCTCAACGCCGAGCGCGACGCGGGCGACGACCGCACCGACACCGGTAGCGAAGGCGGCTACCGCGT
GCCCGGTCTGGAACGCGGTCTGAAGATCCTGACCGAGTTCTCGCCGCGCGAGCCGGTTCTCGGCGCGCCGGAATTGTCCC
GGCGCCTGAAGATTCCTCGTACGACGGTATTCCGCCTGCTGCAAACGCTCGAATCGCTCGGTTTTCTCGAACGCGCCGAC
AAGGACCGCAACTATCGCCTCGGCGTCGCGGTGCTACGGCTCGGCTTCGAATATCTGAGTTCGCTCGAATTGACCGACCT
CGGTTTGCCGATCATCGAAGCGCTGCGCACCGACACCGGCCTGACGAGCCACATCGTGATTCGCGACGGACGCGATGTCG
TGTTTGTCGCCAAGGCGCAAAGCCATGCGCCGATTTTCAGTTCTGTGAAGGTCAACGTCGGCACCCGCCTGCCCGCCTAT
GCGACCACGCACGGCCAGGTGCTGATGGGCGACATGACGCTCGACGATCTGAAGAAGCTCTATCCCGAGCCGGAACTCGA
ACGCTTCACGAAGCAGACGCCTGCCACCGTCGAAGACCTGTACGAACGGATTCTCGACGACGCGCGACGCGGCTTCGCGA
TCAGCGAGTCGTCGTTCGAACGCGGCATTTCGGTGGTGAGCGCGCCGGTGCGTAACGACACGGGCCGCATCGTCGCCGTC
ATCACGACGACGATTCCGCGGCATGAGATCGACGCGTCGCTGCTCGACAGCGGCCTGATCGACAAGGTGCGCCGCGCGGC
CGATGAACTCTCGCAGCGGCTCAACTACCGCCCGAAGACCGGCCCGAACGCGGGCAGCAAATACATGAAGGCACTGGGAC
TCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444I7Z5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y3WNA0

References