Detailed information of TA system TA152250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 442780..443582
• Replicon: plasmid pRL4b2
• Accession: NZ_CP050104
• Organism: Rhizobium leguminosarum bv. trifolii strain 4B

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: HA460_RS25600
• Protein ID: WP_113543032.1
• Coordinates: 442780..443307 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: A0A7Y3SJF5
• Locus tag: HA460_RS25605
• Protein ID: WP_018245607.1
• Coordinates: 443304..443582 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA460_RS25585	438078..439202	-	1125	WP_113543030.1	DNA repair exonuclease	-
HA460_RS25590	439492..440883	-	1392	WP_113543031.1	aldehyde dehydrogenase family protein	-
HA460_RS25595	440885..442531	-	1647	WP_018245609.1	acetolactate synthase large subunit	-
HA460_RS25600	442780..443307	-	528	WP_113543032.1	GNAT family N-acetyltransferase	Toxin
HA460_RS25605	443304..443582	-	279	WP_018245607.1	DUF1778 domain-containing protein	Antitoxin
HA460_RS25610	443737..444615	-	879	WP_113543033.1	exopolysaccharide synthesis protein	-
HA460_RS25615	444939..447053	-	2115	WP_113543034.1	carboxy terminal-processing peptidase	-
HA460_RS25620	447201..447680	-	480	WP_165403373.1	MEKHLA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1232616	1232616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18742.73 Da
• Isoelectric Point: 9.8314

>T152250 WP_113543032.1 NZ_CP050104:c443307-442780 [Rhizobium leguminosarum bv. trifolii]
VTLPVWHEEPIAKSHDRASFGCGDAAMNEFIRRFARQSHEQNAAKTFCAIDKAHPDRILGFYTVAPSAVTHEAVPPKMTR
GLARHEVAGFKLARLATDVKVAGKGLGGQLIAAAALRCLRLASEGGGILLIIDAKSERAAHWYASYGAEPLQGKPLTLVM
PLATFAADFKAKGLL

Nucleotide

• Length: 528 bp

>T152250 NZ_CP066076:943743-943994 [Paraburkholderia ginsengisoli]
ATGTTCACTGACGACGCGTGGGACGATTATCTGTATTGGCAGGAGACCGACCGGAAGGTCCTGCGCAAGATCAATACATT
ACTGGAAGAGTGCCGGCGCGACCCTTATCGGGGGACCGGAAAACCAGAGGCATTGATTGGCAGCTTGAGCGGCTTGTGGT
CGCGCCGTATCACGCTCGCCGATCGGCTGGTCTATCTGCCGAGAGACGGCAAGATCTACGTGATTGCCTGCCGCTTTCAC
TACGACCGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10222.91 Da
• Isoelectric Point: 9.8602

>AT152250 WP_018245607.1 NZ_CP050104:c443582-443304 [Rhizobium leguminosarum bv. trifolii]
MPRTADNKSERYQLRIPPKQKAMIARAAALSNKDMADFILDKIVPEAEAVIQAAEVETVSSRDFIRILDLLERPPKPNAR
LRAAISALPDAL

Nucleotide

• Length: 279 bp

>AT152250 NZ_CP066076:943439-943732 [Paraburkholderia ginsengisoli]
ATGAACGTCCTTACTTACAGCGAGGCGCGTGCCGGCTTTAAGCAAGCGATGGACGACGTCTGCCGGGATCACACGCCTAT
GCTCATCACGAGGCAGAGCGGCGAAAACGTGGTCATGGTGTCTCTCGACGATTTCAATGCAATGCAGGAAACCTTGTATT
TATTGAGTTCGTCGAAGAATGCTCAGCGGCTCGCCAGATCCATCGCGCAATTGAACGCCGGCAGTGCGACCGCGCGCAAA
CTGCTGACCGATGAACCGACAAAAAAGCCGCAACGAGGAGGCCGCACAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Y3SJF5