Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 452112..452902 Replicon plasmid pRL9b4
Accession NZ_CP050098
Organism Rhizobium leguminosarum bv. trifolii strain 9B

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A6G8VU00
Locus tag HA461_RS25865 Protein ID WP_033184561.1
Coordinates 452405..452902 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A6G8VUG8
Locus tag HA461_RS25860 Protein ID WP_033184562.1
Coordinates 452112..452408 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA461_RS25840 447222..447965 - 744 WP_166471985.1 30S ribosomal protein S2 -
HA461_RS25845 448741..449598 - 858 WP_166471883.1 hypothetical protein -
HA461_RS25850 449815..451017 + 1203 WP_166471884.1 hypothetical protein -
HA461_RS25855 451049..451915 - 867 Protein_404 phosphoglycerate dehydrogenase -
HA461_RS25860 452112..452408 + 297 WP_033184562.1 DUF1778 domain-containing protein Antitoxin
HA461_RS25865 452405..452902 + 498 WP_033184561.1 GNAT family N-acetyltransferase Toxin
HA461_RS25870 453336..454595 + 1260 WP_050516897.1 MFS transporter -
HA461_RS25875 454624..455880 + 1257 WP_166471885.1 arsenic transporter -
HA461_RS25880 456000..456110 + 111 Protein_409 CGNR zinc finger domain-containing protein -
HA461_RS25885 456489..456680 + 192 WP_033184625.1 CsbD family protein -
HA461_RS25890 456750..457088 + 339 WP_033184559.1 hypothetical protein -
HA461_RS25895 457322..457726 + 405 WP_033184558.1 host attachment protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..901131 901131


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(28-142)

Antitoxin

Vibrio phage ICP1, Orf50

(16-90)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 17667.25 Da        Isoelectric Point: 8.0964

>T152248 WP_033184561.1 NZ_CP050098:452405-452902 [Rhizobium leguminosarum bv. trifolii]
VTVSVPAPLADHHELAEFHSGVPELDNWLRLRARANQASGASRTFVVCEANRVIAYYALASGAVKQSEAPGRFRRNMPDP
IPVAVLGRLAIDQSYQGRGFGKALVRDAGLRLINAAEILGIRGVLVHAISDGARAFYEAVGFLPSPSDPMMLLVGLHDLN
NALTS

Download         Length: 498 bp

>T152248 NZ_CP066076:c814976-814524 [Paraburkholderia ginsengisoli]
GTGACCCAGCTCTATATGCTGGACACCAGCATCTGCTCATTGGTCGTGCGCGAGCGGCCTCAGTGCATTCTGGCCAGGCT
GCAGGCCTGCAACGCCCAGCAGCGCATTGTCATCTCCGCCATCACGTACGCGGAAATGCGCTTTGCCTCAGCGGGGAAAA
TAGACTCCCCCACGCACGCGCAACTGGTGTCGGCTTTCGTCGCGCGCCTCGACGGCGTTTTGCCGTGGGATGCGATGGCT
GCGGACCGAACCATACGAGTGCGCGTCGAGCTCGCCCATCTGGGTGCCCCAATCAGTGAGAACGATGCGGCGATTGCAGG
TCACGCGCTCGCGGTGGGCGCCATTCTCGTGACCAACAACGCGCGGGAATTCCGACACGTGCGGGGACTTCATATCGAAG
ACTGGGCAAAACCGGCAGTGAGCCAGCGCGCGACCGAGCCGGAGAAGCTATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11038.56 Da        Isoelectric Point: 7.0154

>AT152248 WP_033184562.1 NZ_CP050098:452112-452408 [Rhizobium leguminosarum bv. trifolii]
MPTSHTGKSPDASVALNMRIKPATRNLIDRAAEMLGKTRTDFMLEASERRAEEVLLDRTIFTVSAEVYAEYLARLDAPTQ
PNDRLKRTMSTKAPWDEA

Download         Length: 297 bp

>AT152248 NZ_CP066076:c815233-814973 [Paraburkholderia ginsengisoli]
ATGCGTACTGTTTCCATCTTCAAGAATAGCACCAGCCAGGCAGTTCGCATTCCGAAGGATATGCGGTTCGAAGGTGTGAC
CGAGCTCGAGATCCGGCGTGAAGGCGACACCATCCTGCTGCGGCCCGCACGTCCGAGCTGGATTTCTTTCGCCGATGAGC
CCCGTGCCGATGCCGACTTCATGTCCGGGCGCGCCGCCGTCTTTCGGGCAGGCCGGTATGACCTGCCCGATGCAGTCGAC
GCTCAGGAGTCAGCATCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G8VU00

Antitoxin

Source ID Structure
AlphaFold DB A0A6G8VUG8

References