Detailed information of TA system TA152246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4290040..4290751
• Replicon: chromosome
• Accession: NZ_CP050097
• Organism: Rhizobium leguminosarum bv. trifolii strain 9B

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HA461_RS20965
• Protein ID: WP_130741344.1
• Coordinates: 4290040..4290426 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A444PJK6
• Locus tag: HA461_RS20970
• Protein ID: WP_003556187.1
• Coordinates: 4290428..4290751 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA461_RS20940	4285547..4286428	+	882	WP_130737489.1	polyphosphate kinase 2	-
HA461_RS20945	4286514..4287698	-	1185	WP_166471538.1	regulator	-
HA461_RS20950	4287986..4288945	+	960	WP_166471539.1	nitronate monooxygenase family protein	-
HA461_RS20960	4289336..4289809	+	474	WP_166471540.1	hypothetical protein	-
HA461_RS20965	4290040..4290426	+	387	WP_130741344.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HA461_RS20970	4290428..4290751	+	324	WP_003556187.1	putative addiction module antidote protein	Antitoxin
HA461_RS20975	4290979..4291359	+	381	WP_130737486.1	hypothetical protein	-
HA461_RS20980	4291620..4293110	+	1491	WP_166471541.1	MFS transporter	-
HA461_RS20985	4293185..4293760	+	576	WP_130738143.1	chloramphenicol phosphotransferase	-
HA461_RS20990	4293829..4294674	-	846	WP_130737485.1	methyltransferase domain-containing protein	-
HA461_RS20995	4294949..4295200	+	252	WP_197725686.1	hypothetical protein	-
HA461_RS21000	4295309..4295686	-	378	WP_130737484.1	nuclear transport factor 2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (37-127)

Antitoxin

• Uncharacterized protein HI1420 (15-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14632.56 Da
• Isoelectric Point: 9.3188

>T152246 WP_130741344.1 NZ_CP050097:4290040-4290426 [Rhizobium leguminosarum bv. trifolii]
MVSDDTPRERASGQTSDQQKVDYPREGVNYSSQKYTVLQTVEFQKWLRKLKDRNARDRIVARIVRAEAGNLGDHKFFDGI
GELRLDYGPGYRLYFAMQGSVVVILLCGGDKGSQERDIKRAIEMSKEI

Nucleotide

• Length: 387 bp

>T152246 NZ_CP066073:c515456-515115 [Streptococcus dysgalactiae]
ATGGCCTATAATGTACAAATTACCAAAAGTGCCGAGAGAGATCTAGACAGTATCTATCAGTTCTATCTAGATGAATTTTC
TGAACAAAGTGCTTTGAAAGTTATCAAAAGCCTTCAAGATGCTATCAGCTTTCTTTATGTTTCACCACTTGGCTACACCG
ACTTCGATTCTAAAATCAATCGAAAAATTTATCCACAAGGTAATCTAAGAATGATTCCTTCAAAGCAATACCTTATTTTT
TATCTTATCCGTGAAGAAAGGGTAGATGTTTTAAGAATTATTCATTCTAGAACAGATTATCTCAATAATTTAGAAAATCT
CTTTAAAAATCTAAACCTTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11426.71 Da
• Isoelectric Point: 4.4585

>AT152246 WP_003556187.1 NZ_CP050097:4290428-4290751 [Rhizobium leguminosarum bv. trifolii]
MALEIIPFDATEFLDTEESQTHLIEDAFESGDAGYIAHALGVVAKARGMSQVARDAGVTREALYKSLSEKGDPKLSTLIG
VAAALGYKLTATRVDDSDTHEDARRSA

Nucleotide

• Length: 324 bp

>AT152246 NZ_CP066073:c515733-515446 [Streptococcus dysgalactiae]
ATGAATACACTAGCAAGAGATCGCCAATATAATTTCCGTGTCAATGCGGATATGCTAAATCAGGCTAAGGAAATTTTGGA
GGAAAAACATATGTCTCTACCTGATGCTCTTAATTTATTTGTAGAGCAAGTGGTTGTCACTAAAGACCTCCCTATCAAAA
CACCTGAGCAAGTTCAAGCTGAATCTTTCTTAAATGAATTAATTTCAGAACTTGATGAAGGCTATCAAGATGTTCTAAAA
GGAAATACAAAACCAGCTAATGAGGTTTTTGCAAAATATGGCCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A444PJK6