Detailed information of TA system TA152245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 399348..399910
• Replicon: chromosome
• Accession: NZ_CP050097
• Organism: Rhizobium leguminosarum bv. trifolii strain 9B

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: HA461_RS01830
• Protein ID: WP_166470379.1
• Coordinates: 399665..399910 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: HA461_RS01825
• Protein ID: WP_130703363.1
• Coordinates: 399348..399665 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA461_RS01795	395427..396617	-	1191	WP_166470374.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
HA461_RS01800	397023..397673	+	651	WP_166470375.1	hypothetical protein	-
HA461_RS01805	397735..398025	+	291	WP_033179363.1	BON domain-containing protein	-
HA461_RS01810	398109..398261	-	153	WP_166470376.1	hypothetical protein	-
HA461_RS01815	398379..398621	-	243	WP_166470377.1	hypothetical protein	-
HA461_RS01820	398768..399286	+	519	WP_166470378.1	DUF2937 family protein	-
HA461_RS01825	399348..399665	-	318	WP_130703363.1	BrnA antitoxin family protein	Antitoxin
HA461_RS01830	399665..399910	-	246	WP_166470379.1	BrnT family toxin	Toxin
HA461_RS01840	400223..401386	-	1164	WP_166470380.1	amidohydrolase	-
HA461_RS01845	401544..402677	+	1134	WP_166470381.1	D-alanyl-D-alanine carboxypeptidase	-
HA461_RS01850	402696..403865	+	1170	WP_166470382.1	GTP-binding protein	-
HA461_RS01855	403862..404086	-	225	WP_166470383.1	sulfurtransferase TusA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-103)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9038.65 Da
• Isoelectric Point: 10.0902

>T152245 WP_166470379.1 NZ_CP050097:c399910-399665 [Rhizobium leguminosarum bv. trifolii]
MKLVWDEPKRIANIEKHGLDFAGLTFEFFLSSVVVPAKDGRFKAIGRLADGTMVVIFVNLGSEALSIISMRPARKDERSM
I

Nucleotide

• Length: 246 bp

>T152245 NZ_CP066073:c206298-206044 [Streptococcus dysgalactiae]
ATGTTTAATTTTACTGAAGAGGCTTGGGAAGATTACACTTCTTGGCAAAGAGAAGATAAAAAAATTCTCAAGCGTATTAA
TCGACTGATTGAAGATATCAAACGTCACCCTTTCGAAGGAATCGGAAAACCAGAACCTTTAAAATATCGGTATTCTGGTG
CCTGGTCTCGCCGTATTACAGACGAACACCGTCTCGTTTATACGGTAGAACAAAATGATATCTATTTCTTATCTTTTAGA
GATCACTATAAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11890.75 Da
• Isoelectric Point: 10.5193

>AT152245 WP_130703363.1 NZ_CP050097:c399665-399348 [Rhizobium leguminosarum bv. trifolii]
MIIKARKMTQFQPGRGYSKEDWDAVSDNPPLSKEEMARAKPFKEAFPDVAEKMEKAIAARGRPKLDNPKQPLNIRLDADI
IQFYKATGKGWQSRMNDALRKAAGL

Nucleotide

• Length: 318 bp

>AT152245 NZ_CP066073:c206557-206291 [Streptococcus dysgalactiae]
ATGGAAGCTGTTGTTTATTCAAATTTTAGAAACAATTTAAAAGATTACATGAAAAAGGTAAATGATGAATACGAGCCATT
AATGGTAGTTAATAAAAACCCTGAAGAAAATATCGTGGTCTTGTCTAAAGACAATTGGGATAGTATTCAAGAAACCATTC
GTATCATGAATAACGAATACCTCTCTGATAAAGTTCTATCAGGATTGGAACAAGTCAAACAAAAGAAAGTGACCCAACAC
CAATTATTAGAGGTGGAAGATGTTTAA