Detailed information of TA system TA152242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 427217..427884
• Replicon: plasmid pRL22b6
• Accession: NZ_CP050092
• Organism: Rhizobium leguminosarum bv. trifolii strain 22B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HA462_RS27445
• Protein ID: WP_164037503.1
• Coordinates: 427217..427627 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1M496
• Locus tag: HA462_RS27450
• Protein ID: WP_011649136.1
• Coordinates: 427624..427884 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA462_RS27430	423074..423895	+	822	WP_164037508.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
HA462_RS27435	423917..424831	-	915	WP_131712934.1	hydrogen peroxide-inducible genes activator	-
HA462_RS27440	424975..427155	+	2181	WP_166478641.1	catalase/peroxidase HPI	-
HA462_RS27445	427217..427627	-	411	WP_164037503.1	type II toxin-antitoxin system VapC family toxin	Toxin
HA462_RS27450	427624..427884	-	261	WP_011649136.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HA462_RS27455	428020..428577	-	558	WP_166478642.1	DUF3299 domain-containing protein	-
HA462_RS27460	428685..429653	-	969	WP_166478643.1	zinc-dependent alcohol dehydrogenase family protein	-
HA462_RS27465	429707..430123	+	417	WP_028742372.1	acyl-CoA thioesterase	-
HA462_RS27470	430153..431682	+	1530	WP_166478644.1	AMP-binding protein	-
HA462_RS27475	431660..432865	+	1206	WP_166478645.1	thiolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..826637	826637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-45)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14755.86 Da
• Isoelectric Point: 5.1884

>T152242 WP_164037503.1 NZ_CP050092:c427627-427217 [Rhizobium leguminosarum bv. trifolii]
VITHLIDTNAVIALIGRKSETLLKRVIDSDEGSIGLSTVVMHELYYGAHKSTKISYNLETLRLFVADFSVVGFEQEDALA
AGEIRAALAAKGTPIGPYDVLIAAQARTRDLVLVTNNVGEFGRVDGLRVEDWTVGR

Nucleotide

• Length: 411 bp

>T152242 NZ_CP066071:c2025056-2024853 [Providencia stuartii]
ATGACCAAAACTGATTATCTGATGCGTTTAAGAAAATGCACAACCATCGATACACTTGAGCGTGTTATTGAGAAAAATAA
GTATGAGCTTTCTGAAGATGAGTTAGAATTGTTCTATTCAGCTGCTGACCATCGCCTTGCAGAACTCACTATGAATAAAC
TTTATGACAAAATCCCTGCATCGGTTTGGAAATTTGTTAGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9948.12 Da
• Isoelectric Point: 5.1532

>AT152242 WP_011649136.1 NZ_CP050092:c427884-427624 [Rhizobium leguminosarum bv. trifolii]
MPQYARVFQSGNSQAVRLPKEFRFDVDQVEVTREGDAVILRPRADRGMRWASLHSALERGLSDDFMAEGREQPEAQERPD
LQNLFK

Nucleotide

• Length: 261 bp

>AT152242 NZ_CP066071:c2025504-2025136 [Providencia stuartii]
ATGGATGAATATTCACCCAAAAATTATGATATATCTGAGTTAAAATACTTGTGTAACAGTTTGAATCGAGAAGCAATGCT
AAGCTTACAGAAAACTAATACACACTGGGTCAATGATTTAAGTTCACCACAAAGTGCTAGACTTAATGAACTCATTGAAC
ATATTGCAGCCTTCGTGTGGCAATTTAAGATAAAATATCCCAAAGAGAATCTCGTTATATCATTAGTTGAAGAGTATCTT
GATGAGACTTATGATTTATTCGGAAGCCCTGTAATAACTCTAAGTGAAATTATTGACTGGCAGAGCATGAATCAAAATCT
AGTTTCCGTACTTGATGATGATTTAAAGTGCCCTGCAAGCAAAACATAA

Structures

Antitoxin

• AlphaFold DB: Q1M496