Detailed information of TA system TA152241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 135687..136351
• Replicon: plasmid pRL22b6
• Accession: NZ_CP050092
• Organism: Rhizobium leguminosarum bv. trifolii strain 22B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q1M4X2
• Locus tag: HA462_RS26165
• Protein ID: WP_011648925.1
• Coordinates: 135687..136100 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HA462_RS26170
• Protein ID: WP_166478557.1
• Coordinates: 136097..136351 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA462_RS26150	131299..132312	-	1014	WP_011648922.1	ABC transporter permease	-
HA462_RS26155	132382..134052	-	1671	WP_130788104.1	ABC transporter substrate-binding protein	-
HA462_RS26160	134247..135257	-	1011	WP_166478556.1	GlxA family transcriptional regulator	-
HA462_RS26165	135687..136100	-	414	WP_011648925.1	type II toxin-antitoxin system VapC family toxin	Toxin
HA462_RS26170	136097..136351	-	255	WP_166478557.1	plasmid stabilization protein	Antitoxin
HA462_RS26175	136577..137545	-	969	WP_166478558.1	cyclase family protein	-
HA462_RS26180	137591..138964	-	1374	WP_166478559.1	amidase	-
HA462_RS26185	138984..140198	-	1215	WP_164051301.1	extracellular solute-binding protein	-
HA462_RS26190	140195..141235	-	1041	WP_166478560.1	GTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..826637	826637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14804.20 Da
• Isoelectric Point: 5.7251

>T152241 WP_011648925.1 NZ_CP050092:c136100-135687 [Rhizobium leguminosarum bv. trifolii]
MILLDTNVISEPWKPIPDGAVVAWLDAQAIETLFVSAITIAELRFAIAAMPAGKRQTILRDRLEEEVLPHFAERILPFDL
AASQFYSELMARARVSGKGSARGHIAATAVANGLAVATRDTGPFEAVGLKVINPWLP

Nucleotide

• Length: 414 bp

>T152241 NZ_CP066071:1805830-1806768 [Providencia stuartii]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCAACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGTATGTTACTTGGCAGTGAAGGGATTTCGAGAGAGATTTTGGTGGGTTTTTTAACCTA
TACATTAAGTCACCCTCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTGAATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCCATGATGTTAATTGAACTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGACTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAATTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACACCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9157.27 Da
• Isoelectric Point: 6.2235

>AT152241 WP_166478557.1 NZ_CP050092:c136351-136097 [Rhizobium leguminosarum bv. trifolii]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDIIEAAVRPSERVKLGSLLAAIGGDAELSNSDVEALQQNRNKAPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT152241 NZ_CP066071:1805046-1805837 [Providencia stuartii]
ATGTCATCTAAAATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
CGTGAGCTACTCCCTGGCTCAAAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTTAGTTCTGGTGTGTACTATCGTCCAA
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTGGATGTGCAATTGGGCGTTCCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTGCAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAACAAGTCGTCCTTACCGAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGCGGAAACCATAAGCTTGAAG
TGAATCCCGAGAAGGATTTGAAAAGGATCACGGTTAAAGAGAAAGAGCTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGATGCGATTGGAAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAACGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTAAATTGGGGGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA

Structures

Toxin

• AlphaFold DB: Q1M4X2