Detailed information of TA system TA152240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 77487..78178
• Replicon: plasmid pRL22b6
• Accession: NZ_CP050092
• Organism: Rhizobium leguminosarum bv. trifolii strain 22B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HA462_RS25885
• Protein ID: WP_130824174.1
• Coordinates: 77487..77915 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HA462_RS25890
• Protein ID: WP_130824173.1
• Coordinates: 77912..78178 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA462_RS25855	72584..73387	-	804	WP_166478548.1	crotonase/enoyl-CoA hydratase family protein	-
HA462_RS25860	73425..74066	+	642	WP_130826220.1	TetR/AcrR family transcriptional regulator	-
HA462_RS25865	74323..75162	+	840	WP_003550216.1	transporter substrate-binding domain-containing protein	-
HA462_RS25870	75280..75942	+	663	WP_130824177.1	amino acid ABC transporter permease	-
HA462_RS25875	75948..76607	+	660	WP_130824176.1	amino acid ABC transporter permease	-
HA462_RS25880	76620..77390	+	771	WP_130824175.1	amino acid ABC transporter ATP-binding protein	-
HA462_RS25885	77487..77915	-	429	WP_130824174.1	type II toxin-antitoxin system VapC family toxin	Toxin
HA462_RS25890	77912..78178	-	267	WP_130824173.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HA462_RS25895	78436..79197	+	762	WP_130824172.1	GntR family transcriptional regulator	-
HA462_RS25900	79191..79976	+	786	WP_130824171.1	transporter substrate-binding domain-containing protein	-
HA462_RS25905	80056..80727	+	672	WP_130824170.1	amino acid ABC transporter permease	-
HA462_RS25910	80724..81383	+	660	WP_130824169.1	amino acid ABC transporter permease	-
HA462_RS25915	81361..82086	+	726	WP_012755581.1	amino acid ABC transporter ATP-binding protein	-
HA462_RS25920	82167..82673	-	507	WP_130824168.1	RES family NAD+ phosphorylase	-
HA462_RS25925	82670..83158	-	489	WP_130784826.1	DUF2384 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..826637	826637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-65)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15915.26 Da
• Isoelectric Point: 4.7981

>T152240 WP_130824174.1 NZ_CP050092:c77915-77487 [Rhizobium leguminosarum bv. trifolii]
VRLLLDINVLSEVTRPRPDARVLQWLDSLDEDRSFISTVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVAIAWGDLMGLARRSGRGLSSMDGLIAATAIAHDLTLATRNIKDFEGFGIELVDPWIERP

Nucleotide

• Length: 429 bp

>T152240 NZ_CP066069:1913318-1913659 [Streptococcus dysgalactiae]
ATGGCCTATAATGTACAAATTACCAAAAGTGCCGAGAGAGATCTAGACAGTATCTATCAGTTCTATCTAGATGAATTTTC
TGAACAAAGTGCTTTGAAAGTTATCAAAAGCCTTCAAGATGCTATCAGCTTTCTTTATGTTTCACCACTTGGCTACACCG
ACTTCGATTCTAAAATCAATCGAAAAATTTATCCACAAGGTAATCTAAGAATGATTCCTTCAAAGCAATACCTTATTTTT
TATCTTATCCGTGAAGAAAGGGTAGATGTTTTAAGAATTATTCATTCTAGAACAGATTATCTCAATAATTTAGAAAATCT
CTTTAAAAATCTAAACCTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9907.29 Da
• Isoelectric Point: 7.9904

>AT152240 WP_130824173.1 NZ_CP050092:c78178-77912 [Rhizobium leguminosarum bv. trifolii]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQC
DEPRDLQL

Nucleotide

• Length: 267 bp

>AT152240 NZ_CP066069:1913041-1913328 [Streptococcus dysgalactiae]
ATGAATACACTAGCAAGAGATCGCCAATATAATTTCCGTGTCAATGCGGATATGCTAAATCAGGCTAAGGAAATTTTGGA
GGAAAAACATATGTCTCTACCTGATGCTCTTAATTTATTTGTAGAGCAAGTGGTTGTCACTAAAGACCTCCCTATCAAAA
CACCTGAGCAAGTTCAAGCTGAATCTTTCTTAAATGAATTAATTTCAGAACTTGATGAAGGCTATCAAGATGTTCTAAAA
GGAAATACAAAACCAGCTAATGAGGTTTTTGCAAAATATGGCCTATAA