Detailed information of TA system TA152237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 428343..429013
• Replicon: plasmid pRL23b5
• Accession: NZ_CP050087
• Organism: Rhizobium leguminosarum bv. trifolii strain 23B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HA463_RS32420
• Protein ID: WP_018246604.1
• Coordinates: 428343..428747 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A444HGU5
• Locus tag: HA463_RS32425
• Protein ID: WP_018246605.1
• Coordinates: 428744..429013 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA463_RS32385	423343..423864	+	522	WP_018246596.1	GNAT family N-acetyltransferase	-
HA463_RS32390	423907..424260	+	354	WP_018517210.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HA463_RS32395	424275..424589	-	315	WP_011655201.1	helix-turn-helix domain-containing protein	-
HA463_RS32400	425105..425842	+	738	WP_026188781.1	hypothetical protein	-
HA463_RS32405	425849..426010	-	162	WP_018246599.1	hypothetical protein	-
HA463_RS32410	426828..427388	-	561	WP_018246601.1	hypothetical protein	-
HA463_RS32415	427948..428289	+	342	WP_018246603.1	hypothetical protein	-
HA463_RS32420	428343..428747	-	405	WP_018246604.1	type II toxin-antitoxin system VapC family toxin	Toxin
HA463_RS32425	428744..429013	-	270	WP_018246605.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HA463_RS32430	429235..429453	-	219	WP_003547964.1	hypothetical protein	-
HA463_RS32435	429514..430359	-	846	WP_018517211.1	pirin family protein	-
HA463_RS32440	430455..432215	-	1761	WP_018246607.1	MOSC domain-containing protein	-
HA463_RS32445	432268..432801	-	534	WP_018246608.1	mismatch-specific DNA-glycosylase	-
HA463_RS32450	432944..433429	+	486	WP_026188782.1	DMT family transporter	-
HA463_RS32455	433426..433866	+	441	WP_018246610.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..596075	596075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (18-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14188.56 Da
• Isoelectric Point: 5.1421

>T152237 WP_018246604.1 NZ_CP050087:c428747-428343 [Rhizobium leguminosarum bv. trifolii]
VSRLYMLDTNIVSELARNPMGTITRRIAEVGPDAICVSIITASELRYGCAKKGSPKLLAQIEAILGSIPVLALDLPADAE
YGGIRAELEGAGKPTGPNDLFIAAHASALGAVLVTANMGEFMRIRALQVENWLI

Nucleotide

• Length: 405 bp

>T152237 NZ_CP066065:c1189443-1189072 [Schaalia meyeri]
ATGAGAATCCTCGTTGACACGAACGTGTGGATCGACCACCTGCGCACAACCGAGCCAGTCCTGGTCGATCTGCTCGAACG
TGATCAGGTGTGCGTGCACCAGAGCGTCATCACTGAGCTGGCATTGGGCAACCTGAAAGACCGGTCGATCTTCCTGAAGG
CGTTAGAACGCTTGATGATAGTGCGTAACGTGGATGATCAGGGGGTGCGCTATTTTATCCAGGAGCGGCGGCTGTGGGGT
AGGGGGCTGAGTGCTGTCGATGCGGCAATTCTGGCGAGCGTTGTTGTAACCCCTGGCATGTCACTGTGGACACGCGACAA
GCGCCTGCGTCAGGCCGCGCGCGACGCGGGTGTGCTCGCGGACCTCGACTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10200.61 Da
• Isoelectric Point: 4.5610

>AT152237 WP_018246605.1 NZ_CP050087:c429013-428744 [Rhizobium leguminosarum bv. trifolii]
VVNSRRQEPIPQREAKLFRNNKSQAVRIPADFELPGDRVMIHRDGDRLIIEPVHRKNLLEVLAGLEPLGPEDQFPDVDDT
LLPVEEIDL

Nucleotide

• Length: 270 bp

>AT152237 NZ_CP066065:c1189634-1189440 [Schaalia meyeri]
ATGAGAACGACTGTGACACTCGATGATGACCTGCTCGCCCGCGCTGAGGAGCTCACTGGGATCAGAGAGCGCTCCGTTTT
GATCAGAGACGCGGTTGAGCTCCTCGTCCGTATCGAGAGCGGCCGTCAGTTAGCTGCGCTCGGCGGTAGTGATCCAGGAG
CGGAGGCTGCGCCGCGGAGCCGGGCGCGCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A444HGU5