Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 121073..121845 Replicon plasmid pRL23b5
Accession NZ_CP050087
Organism Rhizobium leguminosarum bv. trifolii strain 23B

Toxin (Protein)


Gene name tacT Uniprot ID A0A2Z4YVU8
Locus tag HA463_RS30965 Protein ID WP_018246328.1
Coordinates 121357..121845 (+) Length 163 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A7Y3WKI8
Locus tag HA463_RS30960 Protein ID WP_026188748.1
Coordinates 121073..121357 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA463_RS30945 116267..118675 + 2409 WP_018246324.1 ABC transporter permease -
HA463_RS30950 118665..119732 + 1068 WP_018246325.1 iron ABC transporter permease -
HA463_RS30955 119840..120589 + 750 WP_018246326.1 sulfite exporter TauE/SafE family protein -
HA463_RS30960 121073..121357 + 285 WP_026188748.1 DUF1778 domain-containing protein Antitoxin
HA463_RS30965 121357..121845 + 489 WP_018246328.1 GNAT family N-acetyltransferase Toxin
HA463_RS30970 122135..122659 + 525 WP_018517806.1 hypothetical protein -
HA463_RS30975 122690..122950 - 261 WP_018246330.1 DUF982 domain-containing protein -
HA463_RS30980 123604..123867 + 264 WP_018246331.1 DUF982 domain-containing protein -
HA463_RS30985 124161..124487 + 327 WP_155256937.1 hypothetical protein -
HA463_RS30990 124524..124736 - 213 WP_018246334.1 hypothetical protein -
HA463_RS30995 125161..125379 + 219 WP_128409058.1 hypothetical protein -
HA463_RS31000 125372..126073 + 702 WP_018246336.1 DUF899 family protein -
HA463_RS31005 126093..126722 - 630 WP_026188749.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..596075 596075


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-142)

Antitoxin

Vibrio phage ICP1, Orf50

(15-86)


Sequences

Toxin        


Download         Length: 163 a.a.        Molecular weight: 17293.88 Da        Isoelectric Point: 7.9271

>T152236 WP_018246328.1 NZ_CP050087:121357-121845 [Rhizobium leguminosarum bv. trifolii]
MLSAPTLLGEAHDLQLFDSGHDSLDEWLRRRARANQVSGASRTYVVCEGERVVGYYCLSSGALAVSDAPGAIRRNMPDPV
PMAVLGRLAIDRNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFYEHFGFAASPANPMALVLSLKAQVG
LK

Download         Length: 489 bp

>T152236 NZ_CP066063:c24866-24771 [Staphylococcus pettenkoferi]
ATGTTGGAAATCCTTGTTCACATCACGACCACAGTCATCAGTGGTTGTATTATTGCGTTATTTACGCATTGGCTGCGTAA
TCGCAAAGACAAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10687.05 Da        Isoelectric Point: 8.6086

>AT152236 WP_026188748.1 NZ_CP050087:121073-121357 [Rhizobium leguminosarum bv. trifolii]
MTSKNATDTHTRAVNLRVREDVRVLIDRAAKAHGKTRSDFMIDASRRAAEDALLDQTLVRVDPDSYKHYLAVLDQPPNSE
GFERLMNAPKPWQP

Download         Length: 285 bp

>AT152236 NZ_CP066063:c24753-24718 [Staphylococcus pettenkoferi]
TACAAAAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z4YVU8

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y3WKI8

References