Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 989900..990480 Replicon plasmid pRL23b7
Accession NZ_CP050086
Organism Rhizobium leguminosarum bv. trifolii strain 23B

Toxin (Protein)


Gene name vapC Uniprot ID A0A444I7Z5
Locus tag HA463_RS29355 Protein ID WP_018245324.1
Coordinates 989900..990283 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7Y3WNA0
Locus tag HA463_RS29360 Protein ID WP_018245323.1
Coordinates 990280..990480 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA463_RS29320 985348..985568 - 221 Protein_896 Gfo/Idh/MocA family oxidoreductase -
HA463_RS29325 985984..987078 + 1095 WP_018245327.1 hypothetical protein -
HA463_RS29330 987375..988427 - 1053 WP_018245326.1 ribosome small subunit-dependent GTPase A -
HA463_RS29335 988462..988638 - 177 WP_018071841.1 hypothetical protein -
HA463_RS29340 988815..989144 + 330 WP_018517229.1 hypothetical protein -
HA463_RS29350 989463..989690 - 228 WP_085990123.1 CopG family transcriptional regulator -
HA463_RS29355 989900..990283 - 384 WP_018245324.1 type II toxin-antitoxin system VapC family toxin Toxin
HA463_RS29360 990280..990480 - 201 WP_018245323.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HA463_RS29365 990645..990884 - 240 WP_155256963.1 hypothetical protein -
HA463_RS29370 991017..991334 + 318 Protein_905 glycerol-3-phosphate dehydrogenase -
HA463_RS29375 991483..991614 - 132 Protein_906 transposase domain-containing protein -
HA463_RS29380 991674..992306 + 633 WP_018245319.1 DUF433 domain-containing protein -
HA463_RS29385 992303..992677 + 375 WP_018245318.1 DUF5615 family PIN-like protein -
HA463_RS29390 992749..994329 - 1581 WP_018245317.1 ABC transporter substrate-binding protein -
HA463_RS29395 994451..995236 + 786 WP_018245316.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..1212571 1212571


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14117.31 Da        Isoelectric Point: 9.1808

>T152235 WP_018245324.1 NZ_CP050086:c990283-989900 [Rhizobium leguminosarum bv. trifolii]
VILADTSIWIDHFRHVDAELRTIIEDDRLLCHPAVVGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASILLDRRAALWTRDKRLQAAAKKAGASLHKPANARN

Download         Length: 384 bp

>T152235 NZ_CP066062:1831745-1831990 [Staphylococcus pettenkoferi]
ATGAACATTCAAGAAGCAACAAAGTTAGCTACGAAAGAACTATTAACAATGACACGAAAAGAATGGAAAGAAAGTCATCA
AGCTCAAGTGTTACCAACGAACGATAGCTTTTTAAACTGCATATTGTCAAGTAATGATGGTAAATGTCTAGTCAGATATT
GGCAACCTTCGGCCGATGACTTAATGGCAGATGATTGGGAGGTTATCAACCCAATCAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7124.23 Da        Isoelectric Point: 11.3760

>AT152235 WP_018245323.1 NZ_CP050086:c990480-990280 [Rhizobium leguminosarum bv. trifolii]
MRSTINLDDTLMERARSLTGTKETAAIVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT152235 NZ_CP066062:c1831995-1831961 [Staphylococcus pettenkoferi]
CACTTCTAAAATTGCTTCAATAATTCCTGGTCTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444I7Z5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y3WNA0

References