Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 359151..359842 Replicon plasmid pRL23b7
Accession NZ_CP050086
Organism Rhizobium leguminosarum bv. trifolii strain 23B

Toxin (Protein)


Gene name vapC Uniprot ID A0A6P0D4X9
Locus tag HA463_RS26390 Protein ID WP_018245712.1
Coordinates 359151..359579 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4R0AD13
Locus tag HA463_RS26395 Protein ID WP_017991284.1
Coordinates 359576..359842 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA463_RS26360 354188..354991 - 804 WP_018245717.1 crotonase/enoyl-CoA hydratase family protein -
HA463_RS26365 355080..355664 + 585 WP_026188704.1 TetR/AcrR family transcriptional regulator -
HA463_RS26370 355937..356776 + 840 WP_018245715.1 transporter substrate-binding domain-containing protein -
HA463_RS26375 356944..357606 + 663 WP_026188703.1 amino acid ABC transporter permease -
HA463_RS26380 357613..358272 + 660 WP_011648868.1 amino acid ABC transporter permease -
HA463_RS26385 358285..359055 + 771 WP_018245713.1 amino acid ABC transporter ATP-binding protein -
HA463_RS26390 359151..359579 - 429 WP_018245712.1 type II toxin-antitoxin system VapC family toxin Toxin
HA463_RS26395 359576..359842 - 267 WP_017991284.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HA463_RS26400 360099..360848 + 750 WP_018245711.1 GntR family transcriptional regulator -
HA463_RS26405 360852..361637 + 786 WP_018245710.1 transporter substrate-binding domain-containing protein -
HA463_RS26410 361718..362389 + 672 WP_018245709.1 amino acid ABC transporter permease -
HA463_RS26415 362386..363045 + 660 WP_166481165.1 amino acid ABC transporter permease -
HA463_RS26420 363023..363748 + 726 WP_018245707.1 amino acid ABC transporter ATP-binding protein -
HA463_RS26425 363897..364268 - 372 WP_018245706.1 type II toxin-antitoxin system RelE/ParE family toxin -
HA463_RS26430 364268..364525 - 258 WP_018245705.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..1212571 1212571


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-66)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15914.18 Da        Isoelectric Point: 4.6772

>T152233 WP_018245712.1 NZ_CP050086:c359579-359151 [Rhizobium leguminosarum bv. trifolii]
VRLLLDTNVLSEVTRPRPDAHVLQWLDSLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVAIAWGDLMGLAKRSGRGLSSMDGLIAATAIAHDLTLATRNIKDFEGFEIELVDPWTERP

Download         Length: 429 bp

>T152233 NZ_CP066062:c507264-507058 [Staphylococcus pettenkoferi]
ATGGAAACCAAACACGTTTATACAGAAACCATTTCTACGCAAGATCAAATCGAAGAATTAAAGCACCTCTTATCTAATAT
GATTGGTGTGAGTGAGGTAGATATTGATGGTGCGAAAGGGGATATTACGGTTACCTTTGAAACGCCTGCGAATTTGAATG
CGATTGAGAAAGAAATTTATGATGCAAGTTATACGATTCTTTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9960.34 Da        Isoelectric Point: 10.0842

>AT152233 WP_017991284.1 NZ_CP050086:c359842-359576 [Rhizobium leguminosarum bv. trifolii]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDLQL

Download         Length: 267 bp

>AT152233 NZ_CP066062:c507570-507286 [Staphylococcus pettenkoferi]
ATGGATCGTGCGCATCATTCTGAACAGACCAAAGCAAATTTGAAGTCTCGATTAAATCGGATTGAAGGACAAGTGAGAGC
CATTAATCGCATGATTGATGAAGATGTCTATTGTGATGATGTACTCACACAAATTAGAGCGACACGTTCAGCGTTAAATA
GTGTTGCCACGCAATTGTTAGACCATCACATGAAAGGTTGCATTCTGAACAAAGTAAATAAAGGTGAAGAAGAGGAAGCG
ATGGAAGAACTGCTCGTCACATTTCAGAAATTGATGAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P0D4X9

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0AD13

References