Detailed information of TA system TA152230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 380875..381410
• Replicon: plasmid pRL31b3
• Accession: NZ_CP050083
• Organism: Rhizobium leguminosarum bv. trifolii strain 31B

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HA464_RS33880
• Protein ID: WP_130657707.1
• Coordinates: 380875..381165 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A4R0A0Z6
• Locus tag: HA464_RS33885
• Protein ID: WP_072642369.1
• Coordinates: 381162..381410 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA464_RS33850	376385..377005	+	621	WP_018069388.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
HA464_RS33855	377002..377931	+	930	WP_018069389.1	phosphatidate cytidylyltransferase	-
HA464_RS33860	377975..378298	-	324	WP_026236977.1	MerR family transcriptional regulator	-
HA464_RS33865	378301..379215	-	915	WP_130689182.1	J domain-containing protein	-
HA464_RS33870	379333..379800	-	468	WP_130689183.1	universal stress protein	-
HA464_RS33875	379961..380863	+	903	WP_130689184.1	NAD(P)-dependent oxidoreductase	-
HA464_RS33880	380875..381165	-	291	WP_130657707.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HA464_RS33885	381162..381410	-	249	WP_072642369.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
HA464_RS33890	381621..382496	+	876	WP_130689185.1	glutathione-dependent disulfide-bond oxidoreductase	-
HA464_RS33895	382581..384095	-	1515	WP_027688759.1	ABC transporter substrate-binding protein	-
HA464_RS33900	384115..385824	-	1710	WP_024320174.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..644077	644077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Antitoxin ParD (4-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11165.75 Da
• Isoelectric Point: 9.5233

>T152230 WP_130657707.1 NZ_CP050083:c381165-380875 [Rhizobium leguminosarum bv. trifolii]
MTGIIFSPAAQTDIEKIWDYTATSWNVDQAERYIQDIRDACHELAEGRRMSRPSDIRKGYRKVSVGSHFLYFKSNDAGQI
IIVRILHRRMDVAKHL

Nucleotide

• Length: 291 bp

>T152230 NZ_CP066060:c2216408-2216157 [Actinomyces oris]
ATGTCGATCAGTGCAAGCGAGGCGCGCAAGACCTTGTACCCGCTCATCGAGCGGGTCAACGCCGATCATGACGCCGTTGA
GATCGTGTCGCGTAAGGGCAGTGCTGTTCTTATGCCCGCTGACGAATACGCCGCTTGGCAGGAGACTGCCTACCTGTTCC
GATCACCTGAGAACGCGCGACGCCTTCTTGATGCGTATGACCGGGCTCGTGCGGGCCACACGGAGATGCATGACCTCGAC
CGGGAGGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8956.96 Da
• Isoelectric Point: 5.0893

>AT152230 WP_072642369.1 NZ_CP050083:c381410-381162 [Rhizobium leguminosarum bv. trifolii]
MARNTSISLGEHFTSFIDTQVQAGRYGSATDVVRAGLRLLEEHEAKVKALQDALIEGEESGPATPFDFDAFNARKRAAFE
AK

Nucleotide

• Length: 249 bp

>AT152230 NZ_CP066060:c2216157-2215903 [Actinomyces oris]
ATGCGTCTCGTATGGGACCCGTCGGCTTGGGAGGACTACACGTACTGGCAGAAGGCGGATCGCCGGATTCTCAAACGGGT
CAACACGCTCATCGATGCGGCCCTTCGCGATCCCTTCGGAGGTATCGGCAAACCCGAGCAGCTCAAGTACGGCGCCTCAG
GTGCGTGGTCGCGGAGGATCACTGACGAGCATCGCCTCGTGTATCTCATCGCTGGCGATGACCTGGTGATTCTCCAAGTT
CGCTACCACTACTGA

Structures

Antitoxin

• AlphaFold DB: A0A4R0A0Z6