Detailed information of TA system TA152228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 273627..274303
• Replicon: plasmid pRL31b5
• Accession: NZ_CP050081
• Organism: Rhizobium leguminosarum bv. trifolii strain 31B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HA464_RS26720
• Protein ID: WP_027688084.1
• Coordinates: 273878..274303 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4Q8ZXM1
• Locus tag: HA464_RS26715
• Protein ID: WP_075224380.1
• Coordinates: 273627..273881 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA464_RS26685	269343..270236	-	894	WP_024321543.1	NmrA family NAD(P)-binding protein	-
HA464_RS26690	270306..270728	-	423	WP_024321542.1	MarR family winged helix-turn-helix transcriptional regulator	-
HA464_RS26695	270768..270962	+	195	Protein_252	hypothetical protein	-
HA464_RS26700	271037..272008	-	972	WP_130663241.1	alpha/beta hydrolase	-
HA464_RS26705	272105..272791	-	687	WP_130663240.1	glutathione S-transferase	-
HA464_RS26710	272896..273471	+	576	WP_029768016.1	TetR/AcrR family transcriptional regulator	-
HA464_RS26715	273627..273881	+	255	WP_075224380.1	plasmid stabilization protein	Antitoxin
HA464_RS26720	273878..274303	+	426	WP_027688084.1	type II toxin-antitoxin system VapC family toxin	Toxin
HA464_RS26725	274749..275537	+	789	WP_024321536.1	siderophore-iron reductase FhuF	-
HA464_RS26730	275654..276331	-	678	Protein_259	phosphopantetheinyl transferase	-
HA464_RS26735	276718..278595	+	1878	WP_130762654.1	methyl-accepting chemotaxis protein	-
HA464_RS26740	278792..279181	+	390	WP_024321534.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..769502	769502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15186.42 Da
• Isoelectric Point: 4.8283

>T152228 WP_027688084.1 NZ_CP050081:273878-274303 [Rhizobium leguminosarum bv. trifolii]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLDDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARAAGRAIGKADAYIAATAAANGFIVATRDSSPFEAAGLKVINPWYAPT

Nucleotide

• Length: 426 bp

>T152228 NZ_CP066060:162-452 [Actinomyces oris]
GTGAGCACCTACCGTCTGACCCCGGCTGCGCGCCGCGATCTGTCACGTATCTGGGACTACAGCGAAGAGCGCTGGGGGCT
GCGGCAGGCCGAGGTCTATCTGCGTAATCTTCAGACGTGTCTGGAAAGACTCGCAGACGATCCTCGTCGAGGGCATCCCA
GAGACGAGGTACGTCCCGGCTACCGAAGCCGCGCCGTCGGAAGCCATGTCGTCTTCTACGTCATCAGTGATGGTGGCGTT
GACGTGATCCGTGTGCTCCACCAACGCATGGACCCCGACAGGCACGTGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9249.43 Da
• Isoelectric Point: 5.6158

>AT152228 WP_075224380.1 NZ_CP050081:273627-273881 [Rhizobium leguminosarum bv. trifolii]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNDDVDNLQLVRDKTPAEP
MSFE

Nucleotide

• Length: 255 bp

>AT152228 NZ_CP066060:3184644-165 [Actinomyces oris]

Structures

Antitoxin

• AlphaFold DB: A0A4Q8ZXM1