Detailed information of TA system TA152226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4588347..4588963
• Replicon: chromosome
• Accession: NZ_CP050078
• Organism: Citrobacter koseri strain NJ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A427P7X6
• Locus tag: NCK_RS21685
• Protein ID: WP_047459878.1
• Coordinates: 4588347..4588721 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: NCK_RS21690
• Protein ID: WP_162790489.1
• Coordinates: 4588718..4588963 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NCK_RS21670 (4585850)	4585850..4586752	+	903	WP_071259455.1	formate dehydrogenase O subunit beta	-
NCK_RS21675 (4586749)	4586749..4587384	+	636	WP_012133917.1	formate dehydrogenase cytochrome b556 subunit	-
NCK_RS21680 (4587381)	4587381..4588310	+	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
NCK_RS21685 (4588347)	4588347..4588721	-	375	WP_047459878.1	type II toxin-antitoxin system VapC family toxin	Toxin
NCK_RS21690 (4588718)	4588718..4588963	-	246	WP_162790489.1	CopG family transcriptional regulator	Antitoxin
NCK_RS21695 (4589168)	4589168..4590097	+	930	WP_047459883.1	alpha/beta hydrolase	-
NCK_RS21700 (4590127)	4590127..4590334	+	208	Protein_4252	transcriptional regulator	-
NCK_RS21705 (4590354)	4590354..4591295	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
NCK_RS21710 (4591340)	4591340..4591777	-	438	WP_047459887.1	D-aminoacyl-tRNA deacylase	-
NCK_RS21715 (4591774)	4591774..4592646	-	873	WP_012133924.1	virulence factor BrkB family protein	-
NCK_RS21720 (4592640)	4592640..4593239	-	600	WP_047459890.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13799.04 Da
• Isoelectric Point: 9.4395

>T152226 WP_047459878.1 NZ_CP050078:c4588721-4588347 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRDEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T152226 NZ_CP066058:c2057350-2057060 [Alcaligenes faecalis]
ATGAAGACTATCCACACCATCGATACCTTCGATCACTGGTTTGGCGGTCTGCGCGACAAGCAGGCCGCTAGACGGATTCA
GGTTCGTATTGACCGAGCTGAAGATGGCAACTTTGGAGACTGCGAGCCTGTCGGCGAAGGAGTCCCCGAAATGCGTATTC
ACTACGGACCTGGCTACCGTGTGTACTTTGTACAACGTGAACTGGAGATCGTGATCTTATTGGCGGATGGCAATAAATCT
ACCCAGAATAAAGATATTAAAACTGCGCTGGAAATCGCGCGGCAACTATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9524.75 Da
• Isoelectric Point: 4.1961

>AT152226 WP_162790489.1 NZ_CP050078:c4588963-4588718 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW
Q

Nucleotide

• Length: 246 bp

>AT152226 NZ_CP066058:c2057047-2056742 [Alcaligenes faecalis]
ATGGGAACCATCAAATTACGCAAATGGGATAGTGCCGAATACCTCCAAACAGAGGAAGACATGGTGCTCTACCTGGAAGC
CTGCATGGAAGAAGCGGGCGACGATGCCGCTTTCATTGCAAAAGCATTAGGAAATATTGCTCGCGCTAAAGGCATGAGCC
AACTGGCCAAAGAAACAGGCTTGGGACGTGAAAGCCTGTACAAAGCCCTGTCTGGCGAAGGCAATCCAAGCTTTGGCACC
ATTCTGAAAGTCATGCACGCTTTGGGAATCAAACTGCAACCGCAGCTTGCTCCCCACAACTCCTAA

Structures

Toxin

• AlphaFold DB: A0A427P7X6