Detailed information of TA system TA152224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3991827..3993377
• Replicon: chromosome
• Accession: NZ_CP050078
• Organism: Citrobacter koseri strain NJ

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: NCK_RS18820
• Protein ID: WP_219936080.1
• Coordinates: 3991827..3993122 (-)
• Length: 432 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: NCK_RS18825
• Protein ID: WP_126291433.1
• Coordinates: 3993126..3993377 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NCK_RS18790 (3986899)	3986899..3988386	+	1488	WP_102987570.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
NCK_RS18795 (3988541)	3988541..3988780	+	240	WP_045330697.1	hypothetical protein	-
NCK_RS18800 (3988820)	3988820..3989137	+	318	Protein_3690	type I restriction-modification system subunit M N-terminal domain-containing protein	-
NCK_RS18805 (3989182)	3989182..3990645	+	1464	WP_258237750.1	MobH family relaxase	-
NCK_RS18810 (3990701)	3990701..3991354	-	654	WP_200028930.1	HNH endonuclease	-
NCK_RS18815 (3991473)	3991473..3991739	-	267	WP_200028928.1	hypothetical protein	-
NCK_RS18820 (3991827)	3991827..3993122	-	1296	WP_219936080.1	type II toxin-antitoxin system HipA family toxin	Toxin
NCK_RS18825 (3993126)	3993126..3993377	-	252	WP_126291433.1	XRE family transcriptional regulator	Antitoxin
NCK_RS18830 (3994188)	3994188..3995051	-	864	WP_200028924.1	hypothetical protein	-
NCK_RS18835 (3995057)	3995057..3996691	-	1635	WP_200028922.1	AAA family ATPase	-
NCK_RS18840 (3997011)	3997011..3997418	-	408	WP_200028920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3955488..3998929	43441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 432 a.a.
• Molecular weight: 49286.60 Da
• Isoelectric Point: 6.6641

>T152224 WP_219936080.1 NZ_CP050078:c3993122-3991827 [Citrobacter koseri]
VEKLTVALNGIIVGTLEKTAGGEMSFYYQRDWLERAGSRAISLSMPLREERYRGEIVYNFFDNLLPDSEQIRSRIQARFH
APTKQPFDLLARIGMDCVGAIQLWPYETDIPSIQRVDAEPLTENEIEHLLQGYKDAPLGMQAEVDDFRISLAGAQEKTAL
FWWNNRWCRPRGSTPTSHIFKLPIGYIANNGIDLRESVENEWLCSKIAAEFGFPVADTRVAYFGQQKILIVERFDRRWAK
DGQWLMRLPTEDFCQALGVAPALKYESDGGPGIARSMDLLLGSQQPLEDRILFFRSQIFFWMLAAIDGHAKNFSVFLEPN
SAYRMAPLYDVMSAYPLMHPKDIPPRKAKMAMALSGRNRHFHWSTIMPRHFLSTAREVSFPVLQVEQLLAEMKQRTESVI
SKVETMLPAGFPEHISEAIFEGLRHQSARIP

Nucleotide

• Length: 1296 bp

>T152224 NZ_CP066058:701007-701303 [Alcaligenes faecalis]
ATGATTAAGAGCTTCCGACACAAGGGTCTTCAGGCTTACTTTGAAACCGGCTCCAAATCCGGCATTCAAGCCAAGCATGC
TGTTCGCCTTCAGATTCAGTTAACGGCCCTGCACTCTGCTACTCAAGCGGAAGATATGAACGCCCCCGGATGGAAGCTCC
ACAAGTTATCTGGCAAAAACCCCAAAGGCCAATCTGTACAAGATCACTACACAATATCGGTTAGCGGAAACTGGCGTCTA
ACCTTTTACTTTGAAGGCGAAGACGCTGTTTTGGTGGACTACCAGGACTATCACTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9472.78 Da
• Isoelectric Point: 9.9484

>AT152224 WP_126291433.1 NZ_CP050078:c3993377-3993126 [Citrobacter koseri]
MNVTSPKILAQAVRDARKQRKLAQREVAESMSMKQSTVSEFENHPEGTRLDTLFKLLAALDLELQVVSRKGKNKSSEGWD
QEW

Nucleotide

• Length: 252 bp

>AT152224 NZ_CP066058:701310-701597 [Alcaligenes faecalis]
ATTATGCGTATGCACAACCCATCCCACCCCGGCCCGGTCTTGCGGGAATACTTGGGCGAACTCAGTGTCACTGAGGCGGC
ATCCCGCCTTGGCGTAACGCGTGCAGCCTTATCGCGCATTCTCAATGGTTCTGCCGGTATTTCGGCAGACATGGCCTTAC
GCCTGCGCGACGCCCTTGGAACCAGTGCTGAAATGTGGATCAACATGCAGTCCCACTACGATCTTTGGCAGGCAGAGCAA
AAGCCAAGGCCCAAAGTCGTGTCCTTTCATGGACCTGCTCATGCCTGA